FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n02_63n1.3520000009e86c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n02_63n1.3520000009e86c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310879
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC25630.8244365171015089No Hit
GGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGG23900.768787856368555No Hit
CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA23830.7665361764545048No Hit
GCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCA20050.6449454610957961No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA19740.6349737357621454No Hit
GGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTA19120.615030285094844No Hit
GTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGA18420.5925134859543424No Hit
CCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG17470.5619549728350902No Hit
GGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGT15550.5001946094782858No Hit
CTCGTCATCACGCCTCAGCCTTGGTTTTCCGGATTTGCCTGGAAAACCAG14880.47864281601523423No Hit
CTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGT13530.4352175605299811No Hit
CCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCC10410.3348569700751739No Hit
GGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAG10040.3229552333866231No Hit
CTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCACCTACA9410.3026901141601716No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC9390.3020467770418716No Hit
CTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCG8850.2846766748477704No Hit
GTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGG8450.27180993248176943No Hit
CGCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGT8320.26762824121281914No Hit
CCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC8060.2592648586749185No Hit
CCTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATG7030.22613299708246618No Hit
CCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAAC6900.22195130581351588No Hit
TGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGT6580.21165791192071515No Hit
CTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAG6470.20811955777006486No Hit
CCCCGTTACATCTTCCGCGCAGGCCGACTCGACCAGTGAGCTATTACGCT6190.19911283811386424No Hit
GAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTAT6140.19750449531811412No Hit
GGCCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCT5950.19139279269426368No Hit
CCCGCCCTACTCATCGAGCTCACAATATGTGCATTTTTGTGTACGGGGCT5950.19139279269426368No Hit
GTGACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCATCAGGTAACATC5860.18849777566191347No Hit
GCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACC5660.18206440447891303No Hit
CCCGTTACATCTTCCGCGCAGGCCGACTCGACCAGTGAGCTATTACGCTT5640.18142106736061298No Hit
CAGCACTTATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCATGA5630.18109939880146295No Hit
GTCCCGCCCTACTCATCGAGCTCACAATATGTGCATTTTTGTGTACGGGG5630.18109939880146295No Hit
CTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGTCAGCAT5600.1801343931240129No Hit
CGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGC5470.17595270185506257No Hit
CGGCTCATCACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTC5380.17305768482271236No Hit
CCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCA5340.17177101058611227No Hit
CTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGT5240.16855432499461204No Hit
CCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGAC5240.16855432499461204No Hit
CGTCGCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCA5190.16694598219886195No Hit
CACTTATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCATGACAA5120.16469430228481177No Hit
GGTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAG5080.16340762804821168No Hit
CCGCCCTACTCATCGAGCTCACAATATGTGCATTTTTGTGTACGGGGCTG4940.15890426822011136No Hit
CCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACT4740.1524708970371109No Hit
GCACTTATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCATGACA4740.1524708970371109No Hit
GCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTC4710.15150589135966083No Hit
GTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTGCGAATGCTG4660.14989754856391072No Hit
GCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGG4590.14764586864986054No Hit
CCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGA4560.14668086297241048No Hit
CTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTG4420.14217750314431016No Hit
GGCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCT4330.13928248611195995No Hit
CTTATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCATGACAACC4320.13896081755280992No Hit
GTCGACTCACCCTGCCCCGATTAACGTTGGACAGGAACCCTTGGTCTTCC4300.1383174804345099No Hit
CTCGGTTGATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGG4280.13767414331620986No Hit
GGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTG4260.1370308061979098No Hit
CCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGAT4260.1370308061979098No Hit
AATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGGAGCCCAG4190.13477912628385966No Hit
GGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAG4150.13349245204725954No Hit
GTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTGC4120.13252744636980945No Hit
AAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGGAGC4080.1312407721332094No Hit
GTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCA4040.1299540978966093No Hit
TCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCAT4010.12898909221915922No Hit
CAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCATCAGGTAACATCAAAT4010.12898909221915922No Hit
CCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCA3970.12770241798255913No Hit
TAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGA3940.12673741230510904No Hit
TGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTG3910.12577240662765898No Hit
ATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCC3900.12545073806850898No Hit
CTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGG3850.12384239527275884No Hit
GCCAGCTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCAC3840.12352072671360884No Hit
CACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAA3830.12319905815445882No Hit
GCCTCGTCATCACGCCTCAGCCTTGGTTTTCCGGATTTGCCTGGAAAACC3790.12191238391785872No Hit
TCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGC3730.11998237256295857No Hit
AGGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAG3680.11837402976720847No Hit
GGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGT3610.11612234985315831No Hit
CGGGCCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGG3550.11419233849825816No Hit
CTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTT3550.11419233849825816No Hit
CCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTA3430.1103323157884579No Hit
GTTGATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTCG3430.1103323157884579No Hit
TCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAA3420.11001064722930785No Hit
GCGACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCATCAGGTAACATC3360.10808063587440774No Hit
CCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGGAGCCCAGAGCCTG3350.10775896731525769No Hit
TGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAA3340.10743729875610769No Hit
GGGAAACAACCCAGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGG3320.10679396163780763No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT3310.10647229307865762No Hit
CCTCGTCATCACGCCTCAGCCTTGGTTTTCCGGATTTGCCTGGAAAACCA3220.10357727604630741No Hit
CGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAG3190.10261227036885733No Hit
CACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACC3170.10196893325055728No Hit
GGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCT3160.10164726469140727No Hit
GCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGA3150.10132559613225724No Hit
CTCAGTTCTCCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCACGACG3110.10003892189565716No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA4300.0121.395352
GATTGGG4000.0116.01
GATCGGG4950.0114.2424241
GAGCGGC2450.0103.571439
GAGGGGC5500.096.227279
AAGGGGC2450.094.693889
ATTGGGA4850.091.185572
GAGAGCG3600.090.6257
AACGGGA2400.090.6252
TCGGGAA1550.088.870973
AGAGCGG4150.087.3493968
GGAGAGG8300.086.47596
GAGAGGG8250.085.2424247
GGAGAGC3950.082.594946
GGGAGAG11200.082.209835
CGGGAAA2450.073.979594
GATTGGA2100.072.51
TTCGGAG809.822543E-1172.499993
GCAGGGG3300.070.30303145
GAAAGGG5450.067.844047
Produced by FastQC (version 0.11.2)