FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n02_62o2.3520000009e985.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n02_62o2.3520000009e985.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226542
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGGG1200.072.51
ATCGGGA1400.067.321432
ACGGGAG1100.065.9090963
CCGACTA400.006044869354.3754
AACGGGA1750.053.857142
GATCGGG2050.053.048781
CGGGAGA2650.049.245284
ACGGAAG752.5442852E-548.3333323
GAGGGGC3750.040.6000029
AGAGCGG1807.094059E-1140.277788
GAGAGGG4650.037.4193577
CGGGAAA1209.531228E-636.254
AGAGGGG5150.035.1941768
AAGGGGC1905.3878466E-934.3421029
TGTACGG1551.4583893E-632.741936145
GAGGGTC1551.4583893E-632.7419369
GGGAGAG6850.030.6934325
GCGTCGG1204.04824E-430.208334145
GAGGGCC1453.478611E-530.09
GAGAGCG1753.7576847E-628.9999987
Produced by FastQC (version 0.11.2)