FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n02_62o1.3520000009e978.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n02_62o1.3520000009e978.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375611
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA2150.067.4418642
GATCGGG2350.061.702121
CGGGAGA3700.058.7837874
CGGACCG400.006047955754.3757
GAACGGG1101.6698323E-952.7272721
AGAGCGG1800.052.361118
GAGGGGC5150.047.864089
GAGAGCG2550.045.4901967
ACGGGAG1307.3287083E-944.6153873
GAGCGGC1651.3606041E-939.5454569
GATCCGG750.001482205838.6666681
CGGGAAA1502.5920599E-838.6666684
AGAGGGG9450.031.4550258
GGAGAGC4150.031.4457826
GATTGGG2558.731149E-1131.274511
GAGAGGG8950.030.7821247
GTCGGGA1453.4846544E-530.02
GGGAGAG12450.029.6987955
TCGGGAA1555.491413E-528.0645163
GGGGAAC2853.2196112E-1027.9824561