FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n02_62n2.3520000009e836.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n02_62n2.3520000009e836.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384398
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC8450.21982424466308356No Hit
GGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGG5620.1462026337285834No Hit
CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA5350.1391786637807689No Hit
CCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG4730.12304954760430596No Hit
GGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTA4680.12174881242878474No Hit
GGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGT4260.11082263695440663No Hit
CTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCACCTACA4040.10509940218211332No Hit
GCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCA3870.10067690258534123No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA5900.0117.96612
GAGGGGC8650.0112.312149
GATCGGG6600.0108.751
GAGAGGG12100.097.066117
GGAGAGG14000.085.446436
AGAGCGG4900.082.857148
GAGCGGC4500.078.944459
GGGAGAG18950.078.430085
ATTGGGA3200.077.031252
GAGAGCG5700.068.684217
TCGGGAG10550.068.033173
AAGGGGC3850.065.9090969
GAGGGTC2000.065.259
GGAGAGC6000.064.041676
GATTGGG3850.064.025981
TCGGGAA2950.063.8983043
GAACGGG4800.061.9270821
AACGGGA4500.061.2222252
CGGGAAA3700.060.743244
AGAGGGG19450.060.0128568
Produced by FastQC (version 0.11.2)