FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n02_62n1.3520000009e829.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n02_62n1.3520000009e829.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345755
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC20620.5963760466226085No Hit
CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA13780.3985481048719469No Hit
GGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGG13390.3872684415265144No Hit
CCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG11720.3389683446370985No Hit
GCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCA11050.31959046145391967No Hit
CCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCC9320.2695550317421296No Hit
GGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTA9220.26666281037150585No Hit
GGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGT8440.24410348368064091No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA8420.24352503940651618No Hit
GTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGA8160.23600526384289452No Hit
CTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCG7920.22906393255339763No Hit
CCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC7140.20650460586253272No Hit
CTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCACCTACA7090.20505849517722086No Hit
CCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATTCAGAACCA6900.19956327457303583No Hit
CTCGTCATCACGCCTCAGCCTTGATTTTCCGGATTTGCCTGGAAAACCAG6640.19204349900941417No Hit
CCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGA6580.19030816618703994No Hit
GGCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCT6510.18828361122760337No Hit
GTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGG6380.18452372344579254No Hit
GCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTC6350.18365605703460544No Hit
CCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTCACCGACGC5940.17179794941504822No Hit
CCTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATG5780.1671703952220503No Hit
CCCCGTTACATCTTCCGCGCAGGCCGACTCGACCAGTGAGCTATTACGCT5700.16485661812555133No Hit
ATCAAATCGTACCCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAG5400.1561799540136802No Hit
GGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAG5350.15473384332836834No Hit
CCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTA5220.15097395554655754No Hit
CTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGTCAGCAT5180.14981706699830805No Hit
CTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGT5110.14779251203887145No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC5040.14576795707943485No Hit
CCCGTTACATCTTCCGCGCAGGCCGACTCGACCAGTGAGCTATTACGCTT4990.14432184639412302No Hit
CCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTCACCGACGCT4890.1414296250234993No Hit
GGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCA4870.14085118074937455No Hit
TCAAATCGTACCCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAGG4610.13333140518575293No Hit
GCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACC4600.13304218304869053No Hit
CCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGT4420.12783618458156787No Hit
CGCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGT4300.12436551893681942No Hit
GCCAGCTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCAC4210.12176251970325809No Hit
GTCGACTCACCCTGCCCCGATTAACGTTGGACAGGAACCCTTGGTCTTCC4200.12147329756619571No Hit
CCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAG3990.11539963268788593No Hit
TCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGC3980.11511041055082356No Hit
GGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGAT3930.11366429986551171No Hit
TGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGT3900.1127966334543246No Hit
CTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGT3860.11163974490607512No Hit
CCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAAC3840.11106130063195035No Hit
GTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCA3590.1038307472053911No Hit
CGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGA3540.10238463652007924No Hit
CTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGG3530.10209541438301688No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTGGG2250.099.8888851
ATTGGGA2600.086.442312
ATCGGGA3300.079.090912
GATCGGG3450.077.7536241
GAGGGGC3450.073.550729
GAGAGGG5400.064.444447
CGGAGTA2650.062.924534
CTATAGG1500.062.833336145
GAGTACC2700.059.0740746
GGAGAGG6200.058.467746
GGAGTAC2900.055.05
AGTACCT3000.053.1666687
GTACCTT3050.052.295088
GGGAGAG8800.051.903415
AAGGGGC2050.049.5121969
CGACCCA604.9405626E-448.3333328
GATTCGG803.7295533E-545.31251
GCAGGGG2750.044.818184145
CCGGAGT3600.044.3055533
GAAAGGG3500.043.57
Produced by FastQC (version 0.11.2)