FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n02_61o2.3520000009e9af.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n02_61o2.3520000009e9af.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617708
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCGTTCTAAGGGCGTTGAAGGTCGATCGTGAGGACGGCTGGAGCGCTT24190.3916089802948966No Hit
GGAGAGCGTTCTAAGGGCGTTGAAGGTCGATCGTGAGGACGGCTGGAGCG23820.3856190951064257No Hit
GCTAAATACTCCCTAGTGACCGATAGTGAACCAGTACCGTGAGGGAAAGG23650.38286698569550665No Hit
CTCGCTTTCGCTGCGGCTCCGTCTCTTCGACTTAACCTCGCATGCAATCG21760.3522700045976416No Hit
CTCGCTTTCGCTGCGGCTCCGTCTCTTCAACTTAACCTCGCATGCAATCG21680.3509748942866209No Hit
CTCGGCTTAGGTCCCGACTAACCCTGGGCGGACGAGCCTTCCCCAGGAAA19050.308398142811814No Hit
GCGGGTTAGAGGGTTCATAACACAAGGGTAGTATCCCACCAGCGCCTCCT18920.30629358855640526No Hit
CTCCATTGCTCAAACAAAAACAACTGGTACAGGAATATCAACCTGTTGTC17240.2790962720249697No Hit
GGCGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACTAA16950.27440149714751955No Hit
GTCGATCGTGAGGACGGCTGGAGCGCTTAGAAGTGAGAATGCCGGTATGA15950.2582126182597603No Hit
GCGTTCTAAGGGCGTTGAAGGTCGATCGTGAGGACGGCTGGAGCGCTTAG14610.23651952055016287No Hit
CGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACTAACA14320.2318247456727127No Hit
CCTCCATTGCTCAAACAAAAACAACTGGTACAGGAATATCAACCTGTTGT14140.22891074747291598No Hit
CCCCATTCGGAAATCTCTGGATCATAGCTTACTTACAGCTCCCCAAAGCA12630.20446554035239953No Hit
CGTCCCTCCATTGCTCAAACAAAAACAACTGGTACAGGAATATCAACCTG12200.19750432243066302No Hit
GGGCGTTGAAGGTCGATCGTGAGGACGGCTGGAGCGCTTAGAAGTGAGAA12090.19572354575300954No Hit
GTAGGAGAGCGTTCTAAGGGCGTTGAAGGTCGATCGTGAGGACGGCTGGA11820.1913525484533145No Hit
GCCGGTATGAGTAGCGAAAGACAGGTGAGAATCCTGTCCACCGTATGACT11790.19086688208668173No Hit
CGCCGGTTCATTCTACAAAAGGCACGCCATCACTCATTAACGAGCTTTGA10910.17662066866545356No Hit
CCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCC9940.1609174561443271No Hit
CAAACACTCAATATCAAACTACAGTAAAGCTCCATGGGGTCTTTCCGTCC9650.15622268126687688No Hit
GTTCTAAGGGCGTTGAAGGTCGATCGTGAGGACGGCTGGAGCGCTTAGAA9630.15589890368912174No Hit
GCCGGTTCATTCTACAAAAGGCACGCCATCACTCATTAACGAGCTTTGAC9610.15557512611136654No Hit
CCTCGACCGATTAGTATTGGTCCGCTCCAACTATCACTAGCCTTCCACTT9540.15444190458922338No Hit
GGGAAACAGCCCAGACCACCAGCTAAGGTCCCAAAATATATGTTAAGTGG9470.15330868306708023No Hit
GGCCTATTCACTGCGGCTGACCGAAGTCAGCACCCCTTCTCCCGAAGTTA8980.1453761324120782No Hit
CACGGTTTCAGGATCTATTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT8870.14359535573442467No Hit
GTGAAAAGCACCCCGGAAGGGGAGTGAAATAGATCCTGAAACCGTGTGCC8720.1411670239012608No Hit
CCCTAGTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGTGAAAAGCAC8530.13809113691258654No Hit
CTAAATACTCCCTAGTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGT8430.1364722490238106No Hit
CCCATTCGGAAATCTCTGGATCATAGCTTACTTACAGCTCCCCAAAGCAT8360.13533902750166746No Hit
GGTCGATCGTGAGGACGGCTGGAGCGCTTAGAAGTGAGAATGCCGGTATG8270.13388202840176913No Hit
CGATAGTGAACCAGTACCGTGAGGGAAAGGTGAAAAGCACCCCGGAAGGG8200.13274880687962595No Hit
GTGGGTTCGGTCCTCCAGTGCGTTTTACCGCACCTTCAACCTGGACATGG7730.12514003380237912No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACTAACACCGAT7710.12481625622462393No Hit
CCGGTTCATTCTACAAAAGGCACGCCATCACTCATTAACGAGCTTTGACT7620.1233592571247256No Hit
TGAAGGTCGATCGTGAGGACGGCTGGAGCGCTTAGAAGTGAGAATGCCGG7590.12287359075809283No Hit
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG7510.12157848044707209No Hit
GGGAAATCTCATCTTGAGGTGGGCTTCACACTTAGATGCTTTCAGCGTTT6940.11235081948104929No Hit
CGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTCGGTCCCTATCCGTCGC6730.10895115491461986No Hit
CCGATAGTGAACCAGTACCGTGAGGGAAAGGTGAAAAGCACCCCGGAAGG6660.10781793339247671No Hit
CTGGGCTACACACGTGCTACAATGGGAAGTACAACGAGTCGCTAGACCGC6660.10781793339247671No Hit
CCCGACTAACCCTGGGCGGACGAGCCTTCCCCAGGAAACCTTAGTTATAC6630.10733226702584393No Hit
CTGAAAAGTAATATCCTATAAAAGATATTGGGTTCCCCCATTCGGAAATC6580.10652282308145596No Hit
CGAAAGGGAAACAGCCCAGACCACCAGCTAAGGTCCCAAAATATATGTTA6390.1034469360927817No Hit
GCCCAGACCACCAGCTAAGGTCCCAAAATATATGTTAAGTGGAAAAGGAT6360.10296126972614893No Hit
GCCGGTATGAGTAGCGAAAGACAGGTGAGAATCCTGTCCACCGTATAACT6350.10279938093727133No Hit
GTCCCGACTAACCCTGGGCGGACGAGCCTTCCCCAGGAAACCTTAGTCAT6340.10263749214839374No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG2050.067.195123
CTCGACC2100.065.595242
GCGGGTT6150.056.585371
GCGCACG4450.052.1348342
ATCGGGA2600.050.192312
TAGAGGG6900.049.384067
GGCGCAC4600.048.8586921
CCACGGT450.00963740448.3333362
GATCGGG3000.048.3333321
CCTCGCT604.943604E-448.3333321
CTCGCTT12500.047.561
CGCACGG5000.046.3999983
TCGCTGC12400.046.1895148
CGCTGCG13350.043.4456949
TTCGCTG13550.042.804437
GATTGGG2550.042.647061
CCGGGTA1201.9718027E-742.2916682
TCGCTTT13900.042.24822
GCTAAAT10700.038.6214941
ATACTCC11050.037.398196
Produced by FastQC (version 0.11.2)