FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n02_61n2.3520000009e90e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n02_61n2.3520000009e90e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280591
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATC5650.2013606993809495Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA3150.0115.079372
AACGGGA3850.097.922082
GAACGGG4000.096.06251
ACGGGAG4750.088.526313
GATCGGG4150.085.602411
AGAGCGG4150.083.855428
GGAGAGG10500.083.5476156
GGGAGAG13650.083.3882755
GAGAGGG10500.081.476197
CGGGAGA9500.077.842114
GAGCGGC3550.075.563389
TCGGGAG5050.068.910893
GAGAGCG4450.066.797757
GAGGGGC11750.061.0851069
AGAGGGG16800.060.4166648
CGGAAAA2400.054.3754
GACGGGA2300.053.586962
TCGGGAA1800.052.361113
ACGGAAG2750.050.0909083
GGAGAGC5950.049.9579856
Produced by FastQC (version 0.11.2)