FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n02_61n1.3520000009e843.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n02_61n1.3520000009e843.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396055
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATC6270.15831134564643798Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA5200.094.8076862
AACGGGA4900.084.336732
GATCGGG6050.082.685941
GAACGGG5450.078.486241
GAGGGGC12900.078.1201559
CGGGAGA11050.076.764714
AGAGCGG7000.076.642858
ATTGGGA4350.075.000012
GATTGGG4650.074.838711
GGGAGAG20300.074.2857065
CGGAAAA2550.071.078444
GGAGAGG16000.069.781256
ACGGAAG3700.068.581083
GAGAGCG6350.065.078747
ACGGGAG5250.064.904763
GAGAGGG17700.063.48877
GAGCGGC6800.061.8382349
AAGGGGC6000.061.6250049
CGGGAAA4900.060.6632654
TCGGGAG9400.056.3031923
Produced by FastQC (version 0.11.2)