FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n02_59n2.3520000009e8c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n02_59n2.3520000009e8c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258319
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA5000.0116.02
GAGGGGC9700.0106.134039
AACGGGA4000.0103.31252
GATCGGG6250.095.1199951
ACGGGAG4850.094.175263
GATTGGG2700.088.6111151
CGGGAGA11000.087.6590964
GAGAGCG4800.087.6041647
GAACGGG5200.086.442311
GGAGAGG13400.085.485086
TCGGGAG7550.085.463583
ATTGGGA2800.080.267862
GGGAGAG18300.079.631155
GAGAGGG14400.078.5416647
CGGAGAG1850.078.378385
AGAGCGG4900.075.459188
ACGGAAG2750.073.8181843
GGAGAGC5850.071.880346
GAGCGGC4400.070.852279
TCGGGAA1700.063.9705853
Produced by FastQC (version 0.11.2)