FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n02_59n1.3520000009e80f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n02_59n1.3520000009e80f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258751
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATC4960.19169008042480995Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC4900.097.653059
ATCGGGA3700.096.013522
GATCGGG3850.090.38961
TCGGGAG4150.089.096383
ATTGGGA2150.080.930232
GAGAGGG7600.078.223697
CGGGAGA6750.075.185194
GGGAGAG11800.071.271195
AACGGGA2800.069.910712
GGAGAGG8750.067.114296
GAACGGG3200.063.43751
GGAGAGC3450.063.0434766
GATTGGG2650.062.924531
AGAGGGG11600.059.3758
GAGAGCG3750.056.066677
TCGGGAA1700.055.4411773
CGGGAAA2900.055.04
AGAGCGG2850.053.4210558
ACGGGAG3400.051.176473
AAGGGGC2300.050.4347849
Produced by FastQC (version 0.11.2)