FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n01_prvabc59b1-1000.3510000009e938.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n01_prvabc59b1-1000.3510000009e938.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2343374
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT321801.3732336366282123TruSeq Adapter, Index 7 (97% over 38bp)
CTTTTCTCTCTTATTTCCCATCATGTTGTACACACAGCTCTGGCACTCTC36570.15605703571004884No Hit
GCAGGATCTGGTGTCATACTGTGGTCCATGGAAGCTAGATGCCGCCTGGG36450.15554495355841622No Hit
GCCACATCCAAGATCAATGACCTTTCCATAGGGCTGCAGGTATCCCCGCT36030.15375266602770193No Hit
GCCGGGGACTGTTTCCAGTGACTTCCGCATCTTTTTCCCATGTGATGTCA31810.13574444369528724No Hit
GCGGGATGGAGCATCCGGGAGACTGCTTGCCTAGCAAAATCATATGCGCA31720.13536038208156273No Hit
GCCGGAGACCCTAGAGACCATAATGCTTTTGGGGTTGCTGGGAACAGTCT30970.1321598686338587No Hit
GCCACATCGAGCCGGGGACTGTTTCCAGTGACTTCCGCATCTTTTTCCCA30460.12998351948941997No Hit
CCGGGGACTGTTTCCAGTGACTTCCGCATCTTTTTCCCATGTGATGTCAC26160.1116339090559168No Hit
CTGGAATCTATGACACGGTCAGCTTTAAAGTTGGCGCCCATCTCTGAAAT25470.1086894366840291No Hit
GCTGCACCTCGCTGTGCCCATCCCAGGCGGCATCTAGCTTCCATGGACCA25230.10766527238076379No Hit
CTTTTCCAACCACATTGGGGATGAATAAGTGTTATATACAGATCATGGAT24900.10625704646377403No Hit
CAGAAAGCAACTTAGTAAGGTCAATGGTGACTGCAGGATCAACTGATCAC24320.1037819827308829No Hit
GCGGCATCTAGCTTCCATGGACCACAGTATGACACCAGATCCTGCTTGAC23790.10152028656117205No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTATT9650.092.409339
CTCTTAT9900.090.075768
TCTCTTA9950.089.623117
GCCGCGT502.2700988E-887.01
GCGTCTA259.388332E-487.04
GCGGGTT703.092282E-1182.857141
CGGGCAT1100.072.51
TGAATCA4300.065.755823
CGGTGCT753.763398E-758.0000041
GAATCAG5000.058.04
GTATTGA651.0941008E-555.769234
GGGCTAT400.006051896654.3751
GCGCGTA400.006051896654.3751
CCGGAGC400.006051896654.3752
TCGGATT400.006051896654.3752
CCCGACA805.8771366E-754.3751
GCCACGT953.1419404E-853.4210551
CGCGCAT858.930001E-751.176471
AGGGGAT2200.049.4318161
CCGGCTA450.00964074148.3333322
Produced by FastQC (version 0.11.2)