FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n01_prvabc59b1-100.3510000009e945.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n01_prvabc59b1-100.3510000009e945.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63136
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT3116149.355359858084135TruSeq Adapter, Index 27 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA16382.5943993917891537TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT12942.0495438418651797TruSeq Adapter, Index 27 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG2800.44348707551951344TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATTTCGTAT2470.39121895590471367TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGACCTCGTAT1260.19956918398378104TruSeq Adapter, Index 27 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTA1030.16313988849467814TruSeq Adapter, Index 27 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC1020.16155600608210846TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTACTCGATCTCGTAT700.11087176887987836TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACTCGATCTCGTAT650.10295235681702991TruSeq Adapter, Index 1 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGCAT640.10136847440446022TruSeq Adapter, Index 27 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC45750.0112.038258
GAGCACA45800.0111.915939
AAGAGCA46250.0110.8270347
TCGGAAG46400.0110.781253
GAAGAGC46350.0110.587926
GATCGGA46550.0110.5800251
CGGAAGA46500.0110.38714
GGAAGAG47050.0109.250795
ATCGGAA47150.0109.172852
CGTACCC400.0060247554.3757
TCAAACG400.0060247554.3752
GTACCCA450.00959765548.3333328
CGTTCGA255.145944E-429.00000245-49
GCCGCAA255.145944E-429.00000235-39
TAATCTA200.006068241329.085-89
TTGAAAA39050.027.6261260-64
GCTTGAA38550.027.57068860-64
GCCGTCT38850.027.46975550-54
CTTGAAA38600.027.45984560-64
TCTGCTT39100.027.4053755-59
Produced by FastQC (version 0.11.2)