FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n01_prvabc59b.3510000009e81f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n01_prvabc59b.3510000009e81f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2981387
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT2535798.50540369297914TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTA103430.346919068205503TruSeq Adapter, Index 6 (97% over 36bp)
GGCGATTCTAGCCTTTTTGAGATTCACGGCAATCAAGCCATCACTGGGTC40970.13741926157187911No Hit
GGAGGGGGCAACCATAGGATTCTCAATGGACATTGACCTGCGGCCAGCCT39950.13399803514270373No Hit
GGAGCATCCGGGAGACTGCTTGCCTAGCAAAATCATATGCGCAAATGTGG35940.12054791947506312No Hit
GCCACATCCAAGATCAATGACCTTTCCATAGGGCTGCAGGTATCCCCGCT34560.11591920136500226No Hit
TGTGAATCAGACTGCGACAGTTCGAGTTTGAAGCGAAAGCTAGCAACAGT34270.11494649973317787No Hit
GGGAGACTTGATCCATACTGGGGAGATGTCAAGCAGGATCTGGTGTCATA31400.10532010772167451No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGGTT1500.062.8331451
GCGGGTT606.8090158E-660.416481
GAGCACA752850.052.676349
CGGTGCT1550.051.4514581
GGGCGCT858.9310197E-751.176311
GATCCGG1152.4865585E-950.434631
GGCGGAT1152.4865585E-950.434631
CGCGCAT1457.2759576E-1249.999851
CGGAAGA795300.049.800874
GAAGAGC801200.049.5336766
TCGGAAG802650.049.4351583
AGAGCAC805050.049.3418168
ATCGGAA816550.048.6025122
AAGAGCA816850.048.5580377
GATCGGG1501.0913936E-1148.333191
CGGGGCC1203.6252459E-948.3331871
AGGGGAC1203.6252459E-948.3331871
GATCGGA825500.048.119481
GGAAGAG851750.046.670545
GGGCTAT657.3390384E-444.6152531
Produced by FastQC (version 0.11.2)