FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n01_negative.3510000009e896.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n01_negative.3510000009e896.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171735
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT10929063.6387457419862TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTA35532.0688852010364807TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT12070.7028270300171776TruSeq Adapter, Index 6 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG9230.5374559641307829TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATTTCGTAT4360.25387952368474687TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGCAT3910.2276763618365505TruSeq Adapter, Index 6 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATGCC2660.1548898011471162TruSeq Adapter, Index 6 (96% over 33bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCT2340.136256441610621Illumina Single End PCR Primer 1 (96% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAACCTCGTAT2160.1257751768713425TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGTATCTCGTAT1950.11354703467551751TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGTGAATCTCGTAT1910.11121786473345562TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCCCGTAT1790.10423035490726992TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGCATCTCGTAT1790.10423035490726992TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA151800.0115.818519
GATCGGA152000.0115.761511
AGAGCAC151900.0115.5036248
TCGGAAG152650.0115.268593
GAAGAGC152500.0115.049186
ATCGGAA153550.0114.5457462
AAGAGCA153700.0114.433967
GGAAGAG153750.0114.208125
CGGAAGA155200.0113.1411064
TCCGGAA350.00356147562.1428573
AGGTTAC902.0361767E-856.388893
TAGGTTA1004.67262E-850.752
GGTTACT1004.67262E-850.754
CTAGGTT1109.894757E-846.136361
CGGAAGG803.721661E-545.31254
TCGTAAG803.721661E-545.31253
TCGGAGG657.319552E-444.6153833
CATCGGA657.319552E-444.6153831
ATCGGAG657.319552E-444.6153832
CAAACGG855.3229585E-542.6470574
Produced by FastQC (version 0.11.2)