FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n01_mr766a1-1000.3510000009e952.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n01_mr766a1-1000.3510000009e952.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1055356
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT12753112.084168754429784TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTA50940.48268072574562515TruSeq Adapter, Index 6 (97% over 36bp)
GTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAA28470.26976678959516975No Hit
GCGGAAGTCACTGGAAACAGTCCTCGGCTTGACGTGGCACTGGATGAGAG23820.22570582817551615No Hit
GCCGGGAAGGCCATTTCGTTTGCTACCACATTGGGAGTGAACAAGTGCCA21130.20021679888113583No Hit
CGCGGAAGTCACTGGAAACAGTCCTCGGCTTGACGTGGCACTGGATGAGA18270.17311693873915532No Hit
GCCGCGCACAGTGATCTGGGCTATTGGATTGAAAGTGAAAAGAATGACAC17630.1670526343717191No Hit
GGGAGATCTTTGCTTCTCTGGCTCGGGTATGAGCACCACCAGTAATAAAA17160.16259916085188314No Hit
GGCCAGTGCATGGCTCATGTGGCTTTCGGAAATTGAACCAGCCAGAATTG15630.148101683223481No Hit
GCCGAGGTAAGATCCTATTGCTACGAGGCATCGATATCGGACATGGCTTC15000.14213213361178598No Hit
GGCGATACTAGCCTTTTTGAGATTTACAGCAATCAAGCCATCACTGGGCC14410.13654160302305574No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT13740.13019303438839594TruSeq Adapter, Index 6 (97% over 36bp)
GCGGGAGTGCATACTACATGTACTTGGATAGGAGCGATGCCGGGAAGGCC13400.12697137269319547No Hit
GCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAGATCACTTTCTTCTA12600.11939099223390022No Hit
CGCGCACAGTGATCTGGGCTATTGGATTGAAAGTGAAAAGAATGACACAT12450.11796967089778235No Hit
GTGTGAGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAG12400.11749589711907642No Hit
GCGGAATAAGGGCATCGGGAAGATGGGCTTTGGAATGGTAACCCTTGGGG12310.11664310431740568No Hit
CTTGTCTTGAAATGATGTCCATAACTGTTTTTCCTCCTTCAGCTGGTCTG12100.11465325444684069No Hit
GCGGGGTCAACCGATCATATGGACCACTTCTCTCTTGGAGTGCTTGTGAT12020.11389521640091116No Hit
GTTTGCTACCACATTGGGAGTGAACAAGTGCCACGTACAGATCATGGACC11930.11304242359924045No Hit
GGCCGAGGTAAGATCCTATTGCTACGAGGCATCGATATCGGACATGGCTT11610.11001027141552235No Hit
GTCAGAGTGCCATTGTCTCGCAACTCCACACATGAGATGTACTGGGTCTC11600.10991551665978117No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGTT350.003566590862.142711
AGAGCAC331200.061.314028
AATCGGA606.805947E-660.416532
GAGCACA342950.059.192179
GAAGAGC348200.058.341346
TCGGAAG349300.058.1991233
CGGAAGA349850.058.1076284
CGCTAAT502.010318E-457.999871
GATCGGA360050.056.4212041
CGGTGCT651.0937012E-555.7691041
ATCGGAA366450.055.5149542
GGAAGAG366850.055.4741825
AAGAGCA374950.054.411137
ATTCGGA400.006051032354.3748742
GCCGCGT553.2191203E-452.727151
GCGGGCA604.9452623E-448.3332251
CGCTGTA450.00963937148.333221
CAGACTG11350.047.2686163
TGCGACA11850.045.2741558
ACTGCGA12300.044.2072146
Produced by FastQC (version 0.11.2)