FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n01_mr766a.3510000009e853.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n01_mr766a.3510000009e853.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1997365
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT36763218.40584970698896TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTA118920.5953844189719956TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT42170.21112816135258206TruSeq Adapter, Index 6 (97% over 36bp)
GGTGCTTACAACACTCCGGGTGTGGACCCTTCCTCACCCAGGTAGCGGAC37940.18995025946684757No Hit
GTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAA37020.1853441909716051No Hit
GTGTGAGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAG32790.16416628908587064No Hit
GGCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAGATCACTTTCTTCT32740.16391595927634658No Hit
GTGCTTACAACACTCCGGGTGTGGACCCTTCCTCACCCAGGTAGCGGACT31530.15785797788586464No Hit
GGGAGATCTTTGCTTCTCTGGCTCGGGTATGAGCACCACCAGTAATAAAA31520.15780791192395982No Hit
GTGAGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTA28960.14499102567632854No Hit
GGCGGCTACCTTATCGGCCTCAGGCCGATAGAGCGAGGCTATGAGGCCAT28670.14353911278108908No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG28140.14088561680013417TruSeq Adapter, Index 6 (97% over 35bp)
GCCGGGAAGGCCATTTCGTTTGCTACCACATTGGGAGTGAACAAGTGCCA28070.1405351550668005No Hit
GGTGGCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAGATCACTTTCT27150.13592908657155803No Hit
GCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAGATCACTTTCTTCTA26960.13497783329536664No Hit
GGCGATACTAGCCTTTTTGAGATTTACAGCAATCAAGCCATCACTGGGCC24540.12286187051440273No Hit
GGACCAGCTAAAGACTTGGGTATGATAAGATCACTTTCTTCTACTCCATC24230.12130982569535363No Hit
GTGGCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAGATCACTTTCTT22260.11144683120010615No Hit
CGCGGAAGTCACTGGAAACAGTCCTCGGCTTGACGTGGCACTGGATGAGA22050.11039544600010515No Hit
GACACATGGAGGCTGAAGAGGGCCCACCTGATTGAGATGAAAACATGTGA20930.10478805826676647No Hit
GTGGACCAGCTAAAGACTTGGGTATGATAAGATCACTTTCTTCTACTCCA19980.10003179188580956No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC685900.086.241078
GAGCACA702850.084.24389
TCGGAAG713600.083.015343
CGGAAGA713200.082.9805764
GAAGAGC715900.082.768896
GATCGGA731150.081.08221
ATCGGAA742050.079.900952
GGAAGAG742800.079.83985
AAGAGCA750250.079.0187
GCCGGAT950.076.315791
CGGTGCT1550.070.161291
GATCGGG1353.6379788E-1253.7037051
GATTCGG1950.048.3333361
TGTGAGT12700.046.2401582
ATTCGGA2050.045.975612
GATCCGG1151.4135367E-744.1304361
CGGGGAG3350.038.9552231
AGGCGAT951.0292592E-438.1578941
ACGGGTG951.0292592E-438.1578941
GGTGCTT15800.034.8734171
Produced by FastQC (version 0.11.2)