FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n01_human-a549.3510000009e96e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n01_human-a549.3510000009e96e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384591
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT322968.397492401018225TruSeq Adapter, Index 2 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA25080.6521213444932408TruSeq Adapter, Index 2 (97% over 37bp)
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT18170.472449953327041No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG15010.3902847440527729No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG14630.3804041176210572No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT11540.30005902374210525No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG9970.2592364355900164No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC9010.23427485302568185No Hit
CGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCG8610.22387419362387573No Hit
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA8320.21633371555756634No Hit
CCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGA7910.20567303967071512No Hit
TGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA7590.19735251214927024No Hit
GCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTAC7120.18513173735214813No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG6800.17681120983070328TruSeq Adapter, Index 2 (97% over 36bp)
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG6420.1669305833989875No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG6270.1630303361233102No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAAC6070.1578300064224072No Hit
GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTA5860.152369660236459No Hit
TCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAG5670.14742934702060112No Hit
CGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCT5490.14274905028978838No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA5420.1409289348944723No Hit
CCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTC5400.14040890192438202No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA5220.13572860519356927No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC5010.1302682590076211No Hit
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC4910.12766809415716956No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG4880.1268880447020341No Hit
CGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAG4840.1258479787618535No Hit
GCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGC4780.1242878798515826No Hit
CGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG4640.12064764906095046No Hit
CGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTC4570.1188275336656344No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC4450.11570733584509259No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC4350.11310717099464107No Hit
CCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCG4280.11128705559932499No Hit
CCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATT4270.11102703911427984No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT4260.11076702262923468No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAA3970.1032265445629253No Hit
CACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATG3890.10114641268256408No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT3880.10088639619751892No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCC259.382325E-487.0145
CCTGCCA2900.077.500011
CTGGTAT406.657976E-572.51
AGCGGTG406.657976E-572.54
GCGCTCA554.0568557E-665.9090962
TGGTATC451.1929277E-464.444442
CGCCCTT350.003564834862.1428571
GCGACTT502.008682E-458.01
TGCCAGT4100.054.8170743
CTGCCAG4100.054.8170742
AGATCAG2050.053.048782
GCCTGTT553.2165067E-452.7272761
CGCTCAG3050.052.2950824
CTCGGCT3450.048.3333321
TCAGACG2300.047.2826045
GCCAGTA4950.045.404044
TGTTACG657.330931E-444.6153833
GGCTCGT3600.044.3055574
GAGCACA117100.043.9581579
CCAGTAG5150.043.6407785
Produced by FastQC (version 0.11.2)