FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n01_67n2.3510000009e8f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n01_67n2.3510000009e8f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453144
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT288496.366408911957347TruSeq Adapter, Index 7 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTA10110.22310788623483924TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATATCGTAT5190.11453312854192044TruSeq Adapter, Index 7 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTT1504.3934644E-529.0000022
GAGCACA167600.028.4636049
AGAGCAC170200.028.1139838
AAGAGCA177950.026.8488337
GAAGAGC178850.026.7137286
AACCGAC1100.00966610926.3636385
CGGAAGA186100.025.7119834
GGAAGAG188450.025.622185
TCGGAAG186850.025.6087763
GGAGCGT2001.0637927E-525.3751
ATCGGAA189900.025.1974722
GATCGGA193200.024.767081
AGCGTTC1902.1905982E-422.8947373
CGCAGTA2903.277837E-722.59
ACCCGCT2903.277837E-722.5145
CGTTCTG2303.137134E-522.0652185
CTCTTAT4006.87578E-1021.758
CTTGAAA45100.021.02660860-64
GCTTGAA47000.020.33085360-64
GCGTTCT2155.05027E-420.2325571
Produced by FastQC (version 0.11.2)