FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n01_67n1.3510000009e889.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n01_67n1.3510000009e889.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331010
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT11691635.320987281351016TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT48111.4534304099574031TruSeq Adapter, Index 27 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA41771.2618954110147729TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATTTCGTAT10200.3081477900969759TruSeq Adapter, Index 27 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG9540.2882088154436422TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGACCTCGTAT4620.13957282257333617TruSeq Adapter, Index 27 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA223150.085.7719049
AGAGCAC225500.084.84598
GAAGAGC227800.083.92566
AAGAGCA227950.083.870377
CGGAAGA231350.082.669114
TCGGAAG232150.082.540383
GGAAGAG232150.082.3530045
GATCGGA233450.082.204961
ATCGGAA234000.081.949792
GATCGGG803.7292055E-545.3125041
GAATCGG750.00148195138.6666681
TCGGGAA800.002034650236.2500043
GATCCGG1051.8510445E-434.5238081
CACGCTT1153.1575645E-431.521741
GAAGCGC950.004722775430.5263186
GCGGGAG950.004722775430.5263181
ATCGGGA1204.0522404E-430.2083342
CGGGAAG1000.00606866629.04
CTTGAAA146350.026.50324460-64
GGGAACC1100.0096624226.3636383
Produced by FastQC (version 0.11.2)