FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n01_66n2.3510000009e91e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n01_66n2.3510000009e91e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583423
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT11996720.562610661561166TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTA48540.831986397519467TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT14240.24407676762829028TruSeq Adapter, Index 6 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG12930.22162307622428323TruSeq Adapter, Index 6 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA320950.060.5390249
AGAGCAC324750.059.8083158
AAGAGCA328650.059.253017
GAAGAGC329900.059.094426
TCGGAAG333750.058.521353
CGGAAGA334200.058.3123864
GATCGGA337750.058.107331
ATCGGAA336850.058.068872
GGAAGAG337050.057.9483765
CGACGGG657.334058E-444.6153871
ATCGGAG1604.5840352E-836.2500042
GCGAGCT900.003627001832.222221
ATCGAAG1504.3956046E-529.0000022
GGTTACT1804.6955556E-628.1944454
GATCGAA1555.4965523E-528.0645141
GATCCGG1306.4796855E-427.8846171
GATTCGG1050.00770586227.6190471
CGAATAC1751.2548489E-424.8571433
TAGGTTA2051.2890652E-524.7560982
GCGAATA1801.5192266E-424.1666662
Produced by FastQC (version 0.11.2)