FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n01_66n1.3510000009e92b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n01_66n1.3510000009e92b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463464
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT5939212.81480330726874TruSeq Adapter, Index 2 (97% over 37bp)
GCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCA23830.5141715429893152No Hit
GGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAG23050.497341756857059No Hit
CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA21980.47425474254742545No Hit
GGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGG21080.4548357585486683No Hit
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC19070.4114666942847772No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA17350.37435485819826353TruSeq Adapter, Index 2 (97% over 37bp)
TGCGAATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACG17140.3698237619318868No Hit
GTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGA17040.367666097043136No Hit
GGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTA16430.35450434122175617No Hit
CCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCC16320.3521309098441303No Hit
GCGAATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACGT15410.332496159356498No Hit
CTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCG15180.3275335301123712No Hit
CCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG15160.327101997134621No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA14860.3206290024683686No Hit
GGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACGTCCGTCGTGA14510.3130771753577408No Hit
GCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTC13930.3005627190029862No Hit
CCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGA13350.2880482626482316No Hit
CTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGTCAGCAT13320.28740096318160635No Hit
CTGCGAATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAAC13250.2858905977594808No Hit
TGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGT12610.27208154247147565No Hit
GGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGT12090.26086168504997154No Hit
CCTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATG11760.25374139091709386No Hit
GTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTAC11590.25007336060621754No Hit
GTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACA11560.2494260611395923No Hit
GGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAAA11480.24769992922859166No Hit
CCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATTCAGAACCA11470.24748416273971657No Hit
GCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCA11430.24662109678421626No Hit
GTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTA11210.24187423402896446No Hit
CTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGT10090.21770838727495556No Hit
GTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCG9950.21468765643070445No Hit
GTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGG9590.20692006283120154No Hit
CCGGAGATGAATTCACGAGGCGCTACCTAAATAGCTTTCGGGGAGAACCA9530.20562546389795108No Hit
CCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAG9330.2013101341204495No Hit
GTCGACTCACCCTGCCCCGATTAACGTTGGACAGGAACCCTTGGTCTTCC9200.19850516976507343No Hit
CCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATTCAGAACCAC8890.19181640860994598No Hit
CCCGTTACATCTTCCGCGCAGGCCGACTCGACCAGTGAGCTATTACGCTT8620.18599071341031884No Hit
GCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCA8580.1851276474548185No Hit
GTGCAATGGCATAAGCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGT8470.18275421607719264No Hit
CCCACATCGTTTCCCACTTAACCATGACTTTGGGACCTTAGCTGGCGGTC8030.1732604905666891No Hit
GGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGG8020.17304472407781402No Hit
CCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGAT7930.1711028256779383No Hit
ATGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTG7900.17045552621131307No Hit
CGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGG7860.16959246025581273No Hit
GGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCA7680.1657086634560613No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC7600.16398253154506068No Hit
GATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTG7530.1624721661229351No Hit
GCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGA7490.1616091001674348No Hit
CCCCGTTACATCTTCCGCGCAGGCCGACTCGACCAGTGAGCTATTACGCT7360.15880413581205877No Hit
TGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAA7290.1572937703899332No Hit
CTGGTATCTTCGACTGATTTCAGCTCCATCCGCAAGGGACTTCACCTACA7280.1570780039010581No Hit
GCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGC7090.1529784406124316No Hit
CTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGT6740.1454266135018038No Hit
GTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGA6550.1413270502131773No Hit
CCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTACCGACGCT6490.14003245127992683No Hit
GAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGT6360.13722748692455078No Hit
TCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCG6280.13550135501355012No Hit
CTTCCCACATCGTTTCCCACTTAACCATGACTTTGGGACCTTAGCTGGCG6180.13334369012479935No Hit
GGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTA6170.13312792363592427No Hit
GTGCTCTACCCCCGGAGATGAATTCACGAGGCGCTACCTAAATAGCTTTC6130.13226485768042395No Hit
GGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTG6080.13118602523604853No Hit
CGCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGT6050.1305387257694233No Hit
CCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACGTCCGTCGT6000.1294598933250479No Hit
CGAATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACGTC5960.12859682736954758No Hit
GAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTAT5940.1281652943917974No Hit
CCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAAC5920.12773376141404724No Hit
CCCATGGCTAGATCACCGGGTTTCGGGTCTATACCCTGCAACTTAACGCC5910.12751799492517218No Hit
CTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGG5750.12406573110317091No Hit
TCAAATCGTACCCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAGG5660.12212383270329519No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTCATTATCTCGTAT5650.1219080662144201TruSeq Adapter, Index 11 (97% over 37bp)
CCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCCG5640.12169229972554503No Hit
CGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACGTCCGTCGTG5630.12147653323666995No Hit
CGTGCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCC5510.11888733537016899No Hit
GGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGG5490.11845580239241882No Hit
CACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCCGG5480.11824003590354376No Hit
GGGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCT5460.11780850292579359No Hit
CAATGGCATAAGCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGCG5440.11737696994804343No Hit
CCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCC5440.11737696994804343No Hit
CGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA5380.11608237101479295No Hit
CAAACTGCGAATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGC5240.11306164017054184No Hit
GCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAG5180.11176704123729135No Hit
GGCCTTCCCACATCGTTTCCCACTTAACCATGACTTTGGGACCTTAGCTG5100.11004090932629071No Hit
CCGTTACATCTTCCGCGCAGGCCGACTCGACCAGTGAGCTATTACGCTTT5080.10960937634854055No Hit
CCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGTCAGCATTCG5000.10788324443753991No Hit
CTACCCCCGGAGATGAATTCACGAGGCGCTACCTAAATAGCTTTCGGGGA4960.1070201784820396No Hit
CGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTC4900.10572557954878913No Hit
CGTCGCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCA4880.10529404657103895No Hit
TCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGC4850.10464674710441373No Hit
ATCACGGGAGACACACGGCGGGTGCTAACGTCCGTCGTGAAGAGGGAAAC4770.10292061519341308No Hit
CAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATG4750.10248908221566291No Hit
GGGAGACACACGGCGGGTGCTAACGTCCGTCGTGAAGAGGGAAACAACCC4690.10119448328241244No Hit
GCGTGCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCC4640.10011565083803703No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGG1900.064.868426
GTAGGGG4000.052.56251
GGAGTAC4250.051.1764765
GGTAGGG2550.051.1764681
GTTCAGT3700.050.945951
GAGTACC4400.049.4318166
CGAACCG4700.049.3617023
GAACCGG2500.049.34
GCTCTAC2400.048.333333
GGCCAGG9050.046.4640921
CGGAGTA4700.046.2765964
GAACGTA1900.045.7894745
GCGAACC2700.045.6481442
GGGTAGG1602.0008883E-1145.31251
AGTGCTC1602.0008883E-1145.31252
CCGAACC2450.044.3877562
AGTACCT4950.043.9393927
GGGAGCG11500.043.52
GGGAACG2000.043.53
ACGTATT2000.043.57
Produced by FastQC (version 0.11.2)