FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n01_65o1.3510000009e995.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n01_65o1.3510000009e995.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425428
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT131273.08559850315447TruSeq Adapter, Index 6 (97% over 36bp)
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC7660.18005396917927358No Hit
GGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGG7230.16994650093552846No Hit
GGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACGTCCGTCGTGA7030.1652453529151819No Hit
GCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCA6400.1504367366510902No Hit
GTCGACTCACCCTGCCCCGATTAACGTTGGACAGGAACCCTTGGTCTTCC6350.14926144964600355No Hit
GCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGC6250.14691087563583027No Hit
TGCGAATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACG5800.1363332925900505No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTA5550.13045685756461728TruSeq Adapter, Index 6 (97% over 36bp)
GGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTA5390.12669593914834001No Hit
GGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAG4920.1156482413005256No Hit
CCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCC4880.11470801169645628No Hit
GCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTC4470.10507065825474582No Hit
GCGAATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACGT4400.10342525644762451No Hit
CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA4300.10107468243745123No Hit
GGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGT4270.10036951023439925No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGG1800.056.388893
GGTAGGG1900.053.4210472
GGGTAGG2000.050.7499961
CCGAACC1604.579124E-836.252
GTGCTCT1751.0068652E-733.1428573
CGGAGTA2051.1354132E-831.8292714
CGAATAC3700.031.3513532
CGTACCT2101.4357283E-831.071433
GGGAGCG4800.030.2083341
GCTCCCA2502.4392648E-929.01
GCTATAG2802.6193447E-1028.4821436
GAGTACC2303.477726E-828.3695666
TCTACCC2054.0299528E-728.2926867
GGAGCGT5250.027.6190471
CTACACG2654.5420165E-927.3584926
CTATAGT2705.5442797E-926.8518527
GGAGTAC2705.5442797E-926.8518525
TACACGC3607.2759576E-1226.1805557
GGCTAGA1958.7390545E-626.0256396
TAGGGGA4200.025.8928594
Produced by FastQC (version 0.11.2)