FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n01_65n2.3510000009e8d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n01_65n2.3510000009e8d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369583
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT359169.717979452518108TruSeq Adapter, Index 6 (97% over 36bp)
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC12380.3349721172240065No Hit
GGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAG12280.33226636506549273No Hit
CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA11750.31792587862536964No Hit
GCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCA11650.31522012646685593No Hit
CCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATTCAGAACCA10160.27490441930500054No Hit
GCGAATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACGT9600.25975220721732334No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTA9580.25921105678562056TruSeq Adapter, Index 6 (97% over 36bp)
GGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGG9500.2570464550588095No Hit
TGCGAATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACG9120.24676459685645716No Hit
CCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG8780.23756503951751026No Hit
TGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGT8750.23675331386995616No Hit
CTGCGAATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAAC8020.2170013231128055No Hit
CCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGA7940.2148367213859945No Hit
GGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTA7620.20617831447875037No Hit
GTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGA7560.20455486318364213No Hit
GTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCG7290.1972493323556549No Hit
GGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACGTCCGTCGTGA7200.1948141554129925No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA6970.18859092544841077No Hit
CCCCGTTACATCTTCCGCGCAGGCCGACTCGACCAGTGAGCTATTACGCT6900.18669689893745112No Hit
ATGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTG6850.18534402285819424No Hit
CCCGTTACATCTTCCGCGCAGGCCGACTCGACCAGTGAGCTATTACGCTT6600.17857964246190977No Hit
GTGCAATGGCATAAGCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGT6490.17560331508754462No Hit
GTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTAC6360.1720858372814767No Hit
GGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGT5920.16018052778401604No Hit
CCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATTCAGAACCAC5710.1544984482511371No Hit
GTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGG5590.15125154566092056No Hit
CTGGTATCTTCGACTGATTTCAGCTCCATCCGCAAGGGACTTCACCTACA5510.14908694393410954No Hit
CCTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATG5180.14015796181101403No Hit
GCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCATCAGGTAACATC4750.12852322752940476No Hit
GATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTG4730.12798207709770199No Hit
GGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCA4640.1255469001550396No Hit
GCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCA4610.12473517450748547No Hit
GCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCA4610.12473517450748547No Hit
GTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACA4520.12229999756482306No Hit
GCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTC4470.12094712148556616No Hit
GGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAAA4370.11824136932705238No Hit
CCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCC4330.11715906846364688No Hit
CGCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGT4330.11715906846364688No Hit
GGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGG4250.11499446673683585No Hit
TCAAATCGTACCCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAGG4200.11364159065757895No Hit
GTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTA4160.11255928979417343No Hit
GCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGA4080.11039468806736241No Hit
CGTCGCTGCCGCAGCTTCGGTGCATGGTTTAGCCCCGTTACATCTTCCGC3990.1079595111247No Hit
TCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCG3980.10768893590884863No Hit
CTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCG3960.10714778547714586No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT3940.10660663504544311TruSeq Adapter, Index 6 (97% over 36bp)
CCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGAT3880.10498318375033483No Hit
TGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAA3880.10498318375033483No Hit
CCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACGTCCGTCGT3830.10363030767107793No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTAGG1450.070.01
CGGAGTA4400.054.3750044
GAGTACC4600.053.586966
GCGAACC2350.052.4468082
GGAGTAC4850.052.3195885
AGTACCT4800.051.3541687
GTACCTT5800.045.08
GGTAGGG2450.044.3877562
ACCGGGG2850.043.2456136
TGGCTAG1557.366907E-1042.0967757
GTAGGGG2950.041.779661
TACCTTT6350.041.1023649
CGGGGGA3050.040.4098368
CGAACCG4050.039.3827173
GAACCGG3200.038.5156254
CCGACTA2052.910383E-1035.3658525
GGGAGCG7000.035.2142832
CCGGAGT6600.035.1515163
GCTCTAC1905.4060365E-934.3421065
CCCATGG1905.4060365E-934.3421063
Produced by FastQC (version 0.11.2)