FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n01_65n1.3510000009e8e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n01_65n1.3510000009e8e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382048
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT65341.7102563028729374TruSeq Adapter, Index 2 (97% over 37bp)
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC36220.9480484127648882No Hit
GCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCA25510.667717145489572No Hit
GGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGG24970.6535827958790519No Hit
CCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG22240.5821258061814222No Hit
CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA21850.5719176647960466No Hit
TGCGAATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACG19380.5072661026886675No Hit
GCGAATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACGT19040.4983666973783399No Hit
GGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAG16920.44287628779629784No Hit
GGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTA15660.4098961387050842No Hit
GTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGA15110.39550004187955445No Hit
GGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGT14410.3771777368288801No Hit
CCCGTTACATCTTCCGCGCAGGCCGACTCGACCAGTGAGCTATTACGCTT14060.368016584303543No Hit
CCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATTCAGAACCA13560.3549292235530614No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA13440.3517882569729458No Hit
GGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACGTCCGTCGTGA13100.3428888516626183No Hit
GCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTC12830.3358216768573582No Hit
TGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGT12790.3347746879973197No Hit
GTCGACTCACCCTGCCCCGATTAACGTTGGACAGGAACCCTTGGTCTTCC12660.3313719742021945No Hit
CCCCGTTACATCTTCCGCGCAGGCCGACTCGACCAGTGAGCTATTACGCT11950.3127879219365106No Hit
CTGCGAATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAAC11850.3101704497864143No Hit
CCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCC11820.30938520814138537No Hit
GGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAAA11630.30441201105620236No Hit
GGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCA11410.29865357232599044No Hit
CCTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATG11150.29184814473574No Hit
GTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTA11110.2908011558757015No Hit
CCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGA10980.28739844208057624No Hit
GTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACA10760.2816400033503644No Hit
GTGCAATGGCATAAGCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGT10330.2703848731049502No Hit
GCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCA9840.25755925956947817No Hit
GTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGG9330.2442101516039869No Hit
CTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCG9180.24028394337884243No Hit
CGCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGT8810.23059929642348606No Hit
GGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGG8450.2211763966831393No Hit
CTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGT8210.21489446352290809No Hit
GCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACC8030.21018301365273473No Hit
GGGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCT8030.21018301365273473No Hit
CGCTGCCGCAGCTTCGGTGCATGGTTTAGCCCCGTTACATCTTCCGCGCA7780.20363933327749392No Hit
CTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGTCAGCAT7670.200760113912388No Hit
GCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCA7480.19578691682720495No Hit
CTGGTATCTTCGACTGATTTCAGCTCCATCCGCAAGGGACTTCACCTACA7390.19343119189211827No Hit
GAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGT7260.19002847809699305No Hit
CCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATTCAGAACCAC7250.1897667308819834No Hit
GCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCATCAGGTAACATC7180.18793450037691597No Hit
GTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTAC7180.18793450037691597No Hit
GTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCG6850.17929684228159812No Hit
GCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGC6850.17929684228159812No Hit
ATGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTG6820.17851160063656923No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC6720.1758941284864729No Hit
GGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTG6680.17484713962643436No Hit
TCAAATCGTACCCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAGG6640.17380015076639585No Hit
CAAACTGCGAATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGC6610.17301490912136694No Hit
TGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAA6570.17196792026132843No Hit
CGTCGCTGCCGCAGCTTCGGTGCATGGTTTAGCCCCGTTACATCTTCCGC6550.17144442583130914No Hit
CGTCGCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCA6370.16673297596113576No Hit
CGAATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACGTC6360.16647122874612613No Hit
CCGTTACATCTTCCGCGCAGGCCGACTCGACCAGTGAGCTATTACGCTTT6270.16411550381103945No Hit
GTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGA6160.1612362844459335No Hit
GGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTA6140.16071279001591424No Hit
GTCGCTGCCGCAGCTTCGGTGCATGGTTTAGCCCCGTTACATCTTCCGCG6050.15835706508082753No Hit
ATCAAATCGTACCCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAG5970.15626308736075048No Hit
GATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTG5920.1549543512857023No Hit
GGTCGACTCACCCTGCCCCGATTAACGTTGGACAGGAACCCTTGGTCTTC5900.15443085685568306No Hit
CCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACGTCCGTCGT5790.1515516374905771No Hit
CGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACGTCCGTCGTG5710.14945765977050005No Hit
CCCACATCGTTTCCCACTTAACCATGACTTTGGGACCTTAGCTGGCGGTC5660.14814892369545188No Hit
GCAATGGCATAAGCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGC5580.14605494597537483No Hit
GCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCA5540.1450079571153363No Hit
GAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTAT5530.14474620990032666No Hit
CGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA5490.14369922104028812No Hit
GGCCTTCCCACATCGTTTCCCACTTAACCATGACTTTGGGACCTTAGCTG5470.14317572661026887No Hit
CCAGCCTACACGCTTAAACCGGGACAACCGTCGCCCGGCCAACATAGCCT5440.14239048496523998No Hit
CCGGAGATGAATTCACGAGGCGCTACCTAAATAGCTTTCGGGGAGAACCA5350.14003476003015328No Hit
GCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAG5270.13794078231007623No Hit
GCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGA5230.13689379345003772No Hit
TCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGC5170.1353233101599799No Hit
CCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCC5140.134538068514951No Hit
GGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGG5120.13401457408493173No Hit
GGGAGACACACGGCGGGTGCTAACGTCCGTCGTGAAGAGGGAAACAACCC5110.1337528268699221No Hit
GTGCTCTACCCCCGGAGATGAATTCACGAGGCGCTACCTAAATAGCTTTC5080.13296758522489321No Hit
GCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGG5060.13244409079487396No Hit
GGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAA5040.13192059636485468No Hit
CCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGAC5030.13165884914984505No Hit
CTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGT4990.13061186028980654No Hit
GGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGG4980.13035011307479688No Hit
TGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTG4970.13008836585978725No Hit
ATCACGGGAGACACACGGCGGGTGCTAACGTCCGTCGTGAAGAGGGAAAC4840.12668565206466204No Hit
GGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGT4830.1264239048496524No Hit
TCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCG4760.12459167434458497No Hit
GGGAAACAACCCAGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGG4760.12459167434458497No Hit
GTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCA4750.12432992712957534No Hit
GTTACATCTTCCGCGCAGGCCGACTCGACCAGTGAGCTATTACGCTTTCT4670.12223594940949829No Hit
CAATGGCATAAGCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGCG4640.1214507077644694No Hit
GCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCG4580.11988022447441159No Hit
CCCATGGCTAGATCACCGGGTTTCGGGTCTATACCCTGCAACTTAACGCC4570.11961847725940197No Hit
CCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGAT4560.11935673004439232No Hit
CTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGT4470.11700100510930564No Hit
GGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAG4420.11569226903425747No Hit
GCGTGCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCC4370.11438353295920932No Hit
CGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGC4370.11438353295920932No Hit
GGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTA4370.11438353295920932No Hit
GCCGCAGCTTCGGTGCATGGTTTAGCCCCGTTACATCTTCCGCGCAGGCC4360.11412178574419969No Hit
CCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACT4240.11098081916408409No Hit
CCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCT4160.10888684144400704No Hit
CTTCCCACATCGTTTCCCACTTAACCATGACTTTGGGACCTTAGCTGGCG4100.10731635815394924No Hit
CAGTGCTCTACCCCCGGAGATGAATTCACGAGGCGCTACCTAAATAGCTT4080.10679286372392999No Hit
CTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAAC4070.10653111650892033No Hit
GCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGT4040.10574587486389146No Hit
CGAACCGACTAATGTTGAAAAATTAGCGGATGACTTGTGGCTGGGGGTGA4030.1054841276488818No Hit
GCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTG4020.10522238043387218No Hit
CCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAAC3950.10339014992880476No Hit
CTACCCCCGGAGATGAATTCACGAGGCGCTACCTAAATAGCTTTCGGGGA3920.10260490828377586No Hit
CCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCC3920.10260490828377586No Hit
ACCGAACCGACTAATGTTGAAAAATTAGCGGATGACTTGTGGCTGGGGGT3920.10260490828377586No Hit
TCTACCCCCGGAGATGAATTCACGAGGCGCTACCTAAATAGCTTTCGGGG3910.10234316106876623No Hit
CGTGCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCC3900.1020814138537566No Hit
AAACTGCGAATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCT3890.10181966663874696No Hit
CCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTACCGACGC3880.10155791942373733No Hit
GGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGAT3870.1012961722087277No Hit
CACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCCGG3860.10103442499371806No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTCC100.0070891595145.06
CGTCGCT2850.063.5964931
ATTAGTA808.069037E-963.43759
GACAAAT851.3023964E-859.7058833
GCGACTA502.008665E-458.01
CGGAGGT400.006048035354.3753
GTAGGGG4050.053.7037051
GGTAGGG2700.053.7037051
GCGAACC3800.053.4210472
GGGTAGG1800.052.3611071
CGGAGTA4900.051.7857134
ACAAATG3100.051.451614
AGTGCTC1850.050.945952
GCGACAA1004.6929927E-850.751
CGACAAA1004.6929927E-850.752
GAACCGG4000.050.754
GCTCCCA5100.049.75491
CCGAACC2050.049.5121962
GAGTACC5250.048.3333326
ACCGGGG4400.047.784096
Produced by FastQC (version 0.11.2)