FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n01_64n2.3510000009e8bf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n01_64n2.3510000009e8bf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433518
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT99132.286640923790938TruSeq Adapter, Index 7 (97% over 38bp)
GCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCA37130.8564811611051906No Hit
GGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGG33660.7764383485806818No Hit
GGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTA26600.6135846723780789No Hit
GTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGA23340.5383859493723444No Hit
CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA22030.5081680576123714No Hit
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC21520.49640384020963374No Hit
GGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGT20040.46226454264874817No Hit
GGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAG19500.4498083124576142No Hit
CCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAAC18550.42789457415839716No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA17050.39329393473858065No Hit
CCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG16240.3746095894518797No Hit
CCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGA16130.37207220922775985No Hit
GTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGG15090.3480824325633538No Hit
CCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCC14840.3423156593267177No Hit
CGCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGT13130.3028709303881269No Hit
GCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGT12800.2952587897157673No Hit
GGCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCT12170.28072652115944435No Hit
CTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCACCTACA12140.28003450837104804No Hit
CTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCG11810.2724223676986884No Hit
GCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGG11610.2678089491093795No Hit
CCTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATG11450.26411821423793247No Hit
CTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGT11340.2615808340138126No Hit
CCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGAT11020.2541993642709184No Hit
CTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGG10620.24497252709230066No Hit
CCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTCACCGACGCT10560.24358850151550798No Hit
GCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAG10470.24151246315031902No Hit
TCAAATCGTACCCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAGG10430.24058977943245724No Hit
GCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTC10360.23897508292619915No Hit
ATCAAATCGTACCCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAG10040.23159361318330496No Hit
TGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGT10020.23113227132437408No Hit
GAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTAT9560.22052140856896368No Hit
GCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACC9540.2200600667100328No Hit
CCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAG9530.21982939578056737No Hit
GGTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAG9320.21498530626179305No Hit
GGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACA9200.21221725510820774No Hit
CTCGTCATCACGCCTCAGCCTTGGTTTTCCGGATTTGCCTGGAAAACCAG8980.20714249465996798No Hit
CTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGT8330.19214888424471419No Hit
ATGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTG8020.18499808543128543No Hit
CGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCT7990.18430607264288912No Hit
CCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCA7810.18015399591251113No Hit
CACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACC7800.17992332498304567No Hit
GGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGT7740.17853929940625302No Hit
GTCGACTCACCCTGCCCCGATTAACGTTGGACAGGAACCCTTGGTCTTCC7660.17669393197052946No Hit
CCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTCACCGACGC7600.1753099063937368No Hit
GCCAGCTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCAC7540.17392588081694416No Hit
TCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAA7490.17277252616961697No Hit
CGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGC7420.17115782966335885No Hit
CTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACC7330.16908179129816986No Hit
CTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGTCAGCAT7330.16908179129816986No Hit
CTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTC7270.1676977657213772No Hit
CTTGCCCCGAGATGAGTTCTCCCTGACTCCTTGAGGGTCCTGAAGGAACG7120.16423770177939556No Hit
GCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGA7030.16216166341420657No Hit
GATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTG6940.16008562504901758No Hit
CGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGG6740.15547220645970872No Hit
CTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTG6730.15524153553024325No Hit
TAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGA6680.15408818088291606No Hit
GGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGAT6600.1522428134471925No Hit
GTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAA6450.14878274950521087No Hit
GTGAAATTGAACTCGCTGTGAAGATGCAGTGTACCCGCGGCAAGACGGAA6430.14832140764627996No Hit
CGTCGCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCA6330.14601469835162556No Hit
GGCCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCT6270.14463067277483288No Hit
CTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACA6240.14393865998643654No Hit
CCGAGATGAGTTCTCCCTGACTCCTTGAGGGTCCTGAAGGAACGTTGAAG6200.14301597626857476No Hit
GGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGG6190.14278530533910933No Hit
CCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGTACCCGCGG6120.14117060883285124No Hit
CTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAG6080.14024792511498946No Hit
CACGCTTAAACCGGGACAACCGTCGCCCGGCCAACATAGCCTTCTCCGTC6040.1393252413971277No Hit
GGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTG5960.13747987396140415No Hit
GTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGCCTTT5900.13609584838461147No Hit
CTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTT5720.13194377165423352No Hit
TGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAA5690.13125175886583718No Hit
CTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGTAC5680.13102108793637174No Hit
CTACACGCTTAAACCGGGACAACCGTCGCCCGGCCAACATAGCCTTCTCC5670.1307904170069063No Hit
CCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGA5660.13055974607744084No Hit
CAAATCGTACCCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAGGC5510.1270996821354592No Hit
TCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGC5460.125946327488132No Hit
GGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCA5320.12271693447561578No Hit
GTGAGGGTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGA5320.12271693447561578No Hit
GTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTGCGAATGCTG5240.12087156703989223No Hit
CAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATG5220.12041022518096134No Hit
CCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCCG5170.11925687053363414No Hit
GTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTT5150.11879552867470325No Hit
GTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCACCTACATAT5140.11856485774523778No Hit
GCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCA5120.11810351588630692No Hit
CCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATTCAGAACCA5040.11625814845058337No Hit
GCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTA5030.11602747752111793No Hit
AGGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAG5030.11602747752111793No Hit
CTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCACCTACATATCAGC5020.11579680659165248No Hit
CACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCCGG4990.11510479380325614No Hit
GGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCA4960.11441278101485981No Hit
CTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGA4910.11325942636753261No Hit
CCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGTACCC4910.11325942636753261No Hit
CCCGAGATGAGTTCTCCCTGACTCCTTGAGGGTCCTGAAGGAACGTTGAA4830.11141405893180907No Hit
AAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGA4810.11095271707287817No Hit
GTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCG4770.1100300333550164No Hit
ATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCC4760.10979936242555095No Hit
GTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTGC4750.10956869149608552No Hit
GTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCA4660.10749265313089652No Hit
GCAGCCAGCTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTT4660.10749265313089652No Hit
GGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTGA4590.10587795662463842No Hit
CGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAAT4590.10587795662463842No Hit
CCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACT4580.10564728569517298No Hit
CCGACACAGGTGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGAACTCGG4510.10403258918891488No Hit
GTAATGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCG4500.10380191825944943No Hit
GTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAG4490.10357124732998399No Hit
GCGGAAGAGATAAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGA4450.10264856361212223No Hit
CAAACCGACACAGGTGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGAAC4420.1019565508237259No Hit
CCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGAC4370.10080319617639867No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC4370.10080319617639867No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGG4150.061.1445771
GAGTACC2000.054.3749966
CGGAGTA2000.054.3749964
AGTACCT2050.053.048787
GGGGAGC11350.052.3788571
GGGAGCG12550.047.370522
GGAGTAC2350.046.2765965
GCGACTA1751.8189894E-1245.5714261
CTCGTCA3700.045.067571
CTACTAG1950.044.6153876
CCTACTA1950.044.6153875
GGCTCCC4400.044.488641
GGTAGGG2600.041.8269231
TAGGGGA5950.041.4285742
TACTAGC2100.041.428577
ACTAGCT2150.040.465128
ATCAAAT4350.040.0000041
TCGTCAT4350.040.0000042
ATCACGC4750.039.684217
GGAGCGT14900.039.412753
Produced by FastQC (version 0.11.2)