FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n01_64n1.3510000009e8a3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n01_64n1.3510000009e8a3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358917
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT342619.54566097454286TruSeq Adapter, Index 2 (97% over 37bp)
GCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCA34020.9478514531214738No Hit
GGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGG30950.862316357263657No Hit
GGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTA26100.7271876227651518No Hit
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC24600.6853952306522121No Hit
GTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGA21430.5970739753201993No Hit
CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA21270.5926161201614858No Hit
GGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGT19950.5558388151020989No Hit
CCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAAC17800.49593638640688514No Hit
GTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGG17200.47921942956170926No Hit
CCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG16990.4733684946658977No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA14720.41012267460164886No Hit
CCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCC14400.4012069642842217No Hit
GTCGACTCACCCTGCCCCGATTAACGTTGGACAGGAACCCTTGGTCTTCC13100.3649868911196739No Hit
CCTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATG12770.35579256485482713No Hit
GGCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCT12220.34046868774674927No Hit
CCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTCACCGACGCT12000.3343391369035181No Hit
CTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGG11880.3309957455344829No Hit
CTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCG11560.32208003521705575No Hit
CCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTCACCGACGC11550.3218014192696361No Hit
CCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAG11190.31177124516253063No Hit
CTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGTCAGCAT10870.30285553484510347No Hit
GCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTC10690.2978404477915507No Hit
CGCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGT10670.2972832158967115No Hit
GGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACA10380.28920335342154313No Hit
GCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGG9850.2744367082083044No Hit
GCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACC9790.2727650125237869No Hit
CTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGT9620.26802854141765364No Hit
CTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGT9370.26106314273216374No Hit
GGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAG9310.2593914470476461No Hit
GAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTAT9030.25159020051989733No Hit
CGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGG8610.23988833072827423No Hit
CCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC8560.23849525099117622No Hit
GGTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAG8480.23626632341181947No Hit
GCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGT7990.22261414198825913No Hit
GGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGAT7920.22066383035632192No Hit
GGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGT7910.22038521440890232No Hit
TAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGA7810.21759905493470635No Hit
TCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAA7790.21704182303986713No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA7450.20756888082760083TruSeq Adapter, Index 2 (97% over 37bp)
GCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAG7430.2070116489327616No Hit
CGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGC7420.206733032985342No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC7410.20645441703792244No Hit
GTCCCGCCCTACTCATCGAGCTCACAATATGTGCATTTTTGTGTACGGGG7290.20311102566888725No Hit
CCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGAT6950.19363808345662087No Hit
CCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGA6930.1930808515617817No Hit
CGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCT6890.19196638777210331No Hit
CCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGA6800.1894588442453269No Hit
CTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTG6790.18918022829790732No Hit
CTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTC6610.18416514124435454No Hit
GTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAA6400.17831420634854298No Hit
GGCCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCT6380.17775697445370378No Hit
CTCGTCATCACGCCTCAGCCTTGGTTTTCCGGATTTGCCTGGAAAACCAG6360.1771997425588646No Hit
CTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACC6260.1744135830846686No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA6250.174134967137249No Hit
CTACACGCTTAAACCGGGACAACCGTCGCCCGGCCAACATAGCCTTCTCC6200.172741887400151No Hit
TCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGC6190.1724632714527314No Hit
GGTCGACTCACCCTGCCCCGATTAACGTTGGACAGGAACCCTTGGTCTTC6140.17107019171563342No Hit
CCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCA5830.1624330973456259No Hit
GGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGG5830.1624330973456259No Hit
CTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGA5800.16159724950336707No Hit
GTGAGGGTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGA5780.16104001760852787No Hit
CTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCACCTACA5630.1568607783972339No Hit
GGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTG5580.1554676986601359No Hit
GTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAG5560.1549104667652967No Hit
CTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTT5430.15128845944884195No Hit
AAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGA5410.15073122755400276No Hit
GACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCG5220.14543752455303038No Hit
CACGCTTAAACCGGGACAACCGTCGCCCGGCCAACATAGCCTTCTCCGTC5220.14543752455303038No Hit
CCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCCG5170.14404444481593237No Hit
GTGAAATTGAACTCGCTGTGAAGATGCAGTGTACCCGCGGCAAGACGGAA5140.14320859697367358No Hit
CGTCGCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCA5080.141536901289156No Hit
ATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCC5060.1409796693943168No Hit
GTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTGCGAATGCTG5020.1398652056046384No Hit
TGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGT4980.13875074181496No Hit
CACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCCGG4940.1376362780252816No Hit
AGGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAG4900.13652181423560322No Hit
CACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACC4880.13596458234076403No Hit
CGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAAT4850.13512873449850524No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA4740.13206395907688964No Hit
GGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTGA4740.13206395907688964No Hit
CGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGA4660.12983503149753287No Hit
GGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCA4490.1250985603913997No Hit
GTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAGGTTA4450.12398409660172129No Hit
CCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGTACCC4440.12370548065430169No Hit
GCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTA4430.1234268647068821No Hit
AAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGGAGC4430.1234268647068821No Hit
CGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAG4350.12119793712752532No Hit
GTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGCCTTT4300.11980485739042732No Hit
CTTGCCCCGAGATGAGTTCTCCCTGACTCCTTGAGGGTCCTGAAGGAACG4260.11869039360074891No Hit
ATGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTG4250.11841177765332932No Hit
CTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGTAC4220.11757592981107054No Hit
CCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGTACCCGCGG4180.11646146602139212No Hit
CCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGG4170.11618285007397253No Hit
TCAAATCGTACCCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAGG4160.11590423412655294No Hit
GTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTGC4150.11562561817913333No Hit
CTCGCCTTAGGGGTCGACTCACCCTGCCCCGATTAACGTTGGACAGGAAC4130.11506838628429414No Hit
ATCAAATCGTACCCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAG4050.11283945870493735No Hit
CCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACT4050.11283945870493735No Hit
GCGGAAGAGATAAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGA4030.11228222681009817No Hit
CCGAGATGAGTTCTCCCTGACTCCTTGAGGGTCCTGAAGGAACGTTGAAG4010.11172499491525897No Hit
ATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAA3960.11033191517816096No Hit
GTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCA3940.10977468328332177No Hit
CCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGAC3930.10949606733590218No Hit
GGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAAC3930.10949606733590218No Hit
CTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACA3920.10921745138848257No Hit
AATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGGAGCCCAG3900.10866021949364338No Hit
GTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTT3820.1064312919142866No Hit
GCCAGCTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCAC3790.1055954440720278No Hit
CCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCA3740.10420236433492981No Hit
CCGCCCTACTCATCGAGCTCACAATATGTGCATTTTTGTGTACGGGGCTG3730.1039237483875102No Hit
TGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTG3730.1039237483875102No Hit
GCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGCC3680.10253066865041222No Hit
GCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3590.10002312512363581No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGG3700.066.621621
CCGCCTA451.1928199E-464.444442
GAGTACC808.067218E-963.4374966
GTTCATT2850.058.508775
TCATTAG3050.057.0491837
CATTAGT3050.057.0491838
ATTAGTA3100.056.1290329
GGCTCCC3100.056.1290321
GGGGAGC9600.055.8854181
GTGCGTC400.00604773954.3749968
GAGGCTC805.8630394E-754.3749962
GGAGTAC1004.6919013E-850.755
CGGAGTA1004.6919013E-850.754
CCCCGGT2450.050.306121
TAGGGGA5150.047.864082
GGGAGCG11400.047.06142
GCGACTA1602.0008883E-1145.3124962
GGTAGGG2250.045.1111151
GGTTCGC3100.044.4354865
TTCATTA3900.040.8974386
Produced by FastQC (version 0.11.2)