FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n01_63n2.3510000009e87c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n01_63n2.3510000009e87c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362781
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT5817616.036120965541194TruSeq Adapter, Index 7 (97% over 38bp)
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC44671.2313213757060044No Hit
GGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGG31320.8633307698032697No Hit
GGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTA27920.7696103158654946No Hit
GCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCA27600.7607895672595864No Hit
CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA25510.7031790529272481No Hit
CCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG23620.6510815064736025No Hit
GGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGT22140.6102855441712769No Hit
CTCGTCATCACGCCTCAGCCTTGGTTTTCCGGATTTGCCTGGAAAACCAG21300.5871310790807678No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA21060.5805155176263366No Hit
GTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGA18150.5003018349913584No Hit
CTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGT16550.45619809196181715No Hit
GGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAG14130.38949118062963606No Hit
CTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCACCTACA11510.3172713014187623No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATATCGTAT11040.3043158269038346TruSeq Adapter, Index 7 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTA9650.2660007001469206TruSeq Adapter, Index 7 (97% over 38bp)
CCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAAC9490.26159032584396646No Hit
CCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCC8450.23292289287476464No Hit
GTGACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCATCAGGTAACATC8310.22906381535967982No Hit
GGTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAG8270.22796122178394126No Hit
CAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCATCAGGTAACATCAAAT7900.21776223120835986No Hit
GAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTAT7720.21280056011753648No Hit
GCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGG7510.20701194384490917No Hit
CTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCG7470.20590935026917065No Hit
CGCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGT7340.20232592114802045No Hit
CCCCGTTACATCTTCCGCGCAGGCCGACTCGACCAGTGAGCTATTACGCT7120.19626165648145852No Hit
TGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGT6650.18330618196653078No Hit
TCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCAT6510.1794471044514459No Hit
ATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCC6310.17393413657275325No Hit
GCCTCGTCATCACGCCTCAGCCTTGGTTTTCCGGATTTGCCTGGAAAACC6170.1700750590576684No Hit
GCACTTATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCATGACA6150.16952376226979912No Hit
CTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCACCTACATATCAGC5890.16235690402749867No Hit
CTCGGTTGATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGG5800.159876068482087No Hit
CCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGAT5700.15711958454274066No Hit
CAGCACTTATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCATGA5680.1565682877548714No Hit
CCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGAC5670.15629263936093676No Hit
CTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGG5660.15601699096700214No Hit
CTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAG5590.15408745220945969No Hit
CCCGTTACATCTTCCGCGCAGGCCGACTCGACCAGTGAGCTATTACGCTT5580.15381180381552506No Hit
CGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGC5570.15353615542159044No Hit
TGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTG5570.15353615542159044No Hit
GTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTGCGAATGCTG5550.1529848586337212No Hit
CGGCTCATCACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTC5480.15105531987617873No Hit
GGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGT5320.1466449455732246No Hit
CCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACT5230.1441641100278129No Hit
GGCCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCT5130.14140762608846658No Hit
GTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCA5130.14140762608846658No Hit
CCCCCCTCAGTTCTCCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCA5010.138099845361251No Hit
GCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACC5000.13782419696731638No Hit
GTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTGC4920.1356190098158393No Hit
CTTATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCATGACAACC4860.13396511945223152No Hit
CCTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATG4840.13341382266436225No Hit
CCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGA4760.13120863551288517No Hit
GCCAGCTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCAC4730.1303816903310813No Hit
CCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCA4670.1287277999674735No Hit
CTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACC4650.12817650317960422No Hit
GCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGA4520.124593074058454No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGATTCCATCTCGTAT4510.12431742566451937TruSeq Adapter, Index 6 (97% over 37bp)
CACTTATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCATGACAA4470.12321483208878083No Hit
GGCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCT4420.12183659011910768No Hit
CAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCG4350.11990705136156524No Hit
ATCAAATCGTACCCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAG4340.11963140296763061No Hit
CCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTA4300.11852880939189209No Hit
TGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTG4260.11742621581615355No Hit
TGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAA4090.11274019311926478No Hit
GTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGCCTTT4070.11218889633139552No Hit
GCGACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCATCAGGTAACATC4060.1119132479374609No Hit
CCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGA4050.11163759954352626No Hit
GCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAG3980.10970806078598383No Hit
CTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGT3980.10970806078598383No Hit
CTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGTCAGCAT3980.10970806078598383No Hit
CGTCGCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCA3950.10888111560417993No Hit
TCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAA3940.1086054672102453No Hit
GTGAGGGTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGA3930.10832981881631068No Hit
CTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTG3900.10750287363450677No Hit
CGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAG3870.10667592845270286No Hit
CTCAGTTCTCCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCACGACG3850.1061246316648336No Hit
GCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTC3760.10364379611942191No Hit
GGACGTGCTAATCTGCGATAAGCGTCGGTGAGGTGATATGAACCGTTATA3750.10336814772548729No Hit
TCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGC3740.10309249933155265No Hit
CCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCTGACCACCTGTGTCG3730.10281685093761803No Hit
CGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAAT3710.10226555414974874No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGAGC5900.073.7283251
GAGCACA150150.066.6812069
AGAGCAC150950.066.375858
AAGAGCA153300.065.500217
GAAGAGC153700.065.329756
TCGGAAG157550.063.825343
GGAAGAG159950.062.8676645
ATCGGAA160500.062.6974032
GATCGGA161950.062.0465161
GGGAGCG7050.060.673362
ATCACGC5600.056.963917
CGTCATC5600.056.963913
TCGTCAT5800.054.9996342
CATCACG5950.053.6130876
CCGCAGC953.132118E-853.4206966
CGGAAGA189150.053.277474
TCACGCC6050.052.7269258
CCGCCTA553.2163688E-452.7269252
ACCTCGA1550.051.451274
GTCATCA6500.049.0765954
Produced by FastQC (version 0.11.2)