FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n01_63n1.3510000009e86f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n01_63n1.3510000009e86f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310879
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT237547.640914954049646TruSeq Adapter, Index 2 (97% over 37bp)
GGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGG26450.8508133389518108No Hit
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC25580.8228281743057588No Hit
GCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCA25060.8061014092299577No Hit
CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA23060.741767697399953No Hit
GGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTA21560.6935174135274497No Hit
GTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGA20850.670678945827798No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA18900.6079535767935434No Hit
GGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGT17300.5564866073295398No Hit
CCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG16560.532683133952438No Hit
CTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGT16500.5307531225975379No Hit
CTCGTCATCACGCCTCAGCCTTGGTTTTCCGGATTTGCCTGGAAAACCAG15120.48636286143483476No Hit
GGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAG11480.3692755059042264No Hit
CCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCC8740.28113832069712014No Hit
CCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAAC8720.2804949835788201No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC8720.2804949835788201No Hit
GTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGG8610.27695662942816984No Hit
CGCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGT8290.2666632355353691No Hit
CTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCACCTACA8130.26151653858896867No Hit
TGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGT7700.24768479054551767No Hit
GTGACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCATCAGGTAACATC7250.2332097053837667No Hit
CCTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATG7150.22999301979226644No Hit
CTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCG7130.2293496826739664No Hit
CCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC6920.2225946429318159No Hit
GAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTAT6880.2213079686952158No Hit
CTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGTCAGCAT6250.20104284946876436No Hit
GGTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAG6170.1984695009955642No Hit
CTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAG6060.19493114684491392No Hit
CGGCTCATCACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTC5860.18849777566191347No Hit
CAGCACTTATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCATGA5710.18367274727466312No Hit
CCCGCCCTACTCATCGAGCTCACAATATGTGCATTTTTGTGTACGGGGCT5710.18367274727466312No Hit
CCCGTTACATCTTCCGCGCAGGCCGACTCGACCAGTGAGCTATTACGCTT5710.18367274727466312No Hit
GCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACC5450.17530936473676254No Hit
CCCCGTTACATCTTCCGCGCAGGCCGACTCGACCAGTGAGCTATTACGCT5340.17177101058611227No Hit
AATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGGAGCCCAG5330.17144934202696227No Hit
CACTTATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCATGACAA5300.17048433634951218No Hit
CTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGG5250.16887599355376207No Hit
GGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAG5250.16887599355376207No Hit
GCACTTATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCATGACA5230.16823265643546204No Hit
GTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTGCGAATGCTG5200.16726765075801195No Hit
GCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGG5190.16694598219886195No Hit
AAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGGAGC5170.1663026450805619No Hit
GGCCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCT5060.16276429092991163No Hit
GTCCCGCCCTACTCATCGAGCTCACAATATGTGCATTTTTGTGTACGGGG5040.1621209538116116No Hit
CGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGC5030.16179928525246157No Hit
CTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGT4880.15697425686521121No Hit
CCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGAT4870.1566525883060612No Hit
GGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGT4860.15633091974691118No Hit
CAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCATCAGGTAACATCAAAT4850.15600925118776116No Hit
CTTATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCATGACAACC4830.1553659140694611No Hit
CCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACT4830.1553659140694611No Hit
GTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTGC4820.15504424551031107No Hit
ATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCC4610.1482892057681606No Hit
CCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGAC4560.14668086297241048No Hit
CTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTG4530.14571585729496042No Hit
GGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTG4440.14282084026261022No Hit
TGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTG4430.1424991717034602No Hit
TAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGA4260.1370308061979098No Hit
GCCTCGTCATCACGCCTCAGCCTTGGTTTTCCGGATTTGCCTGGAAAACC4250.13670913763875978No Hit
CGTCGCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCA4230.13606580052045972No Hit
CCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGA4210.1354224634021597No Hit
GGGAAACAACCCAGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGG4150.13349245204725954No Hit
CCGCCCTACTCATCGAGCTCACAATATGTGCATTTTTGTGTACGGGGCTG4100.13188410925150945No Hit
AGGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAG4040.1299540978966093No Hit
CGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAG4010.12898909221915922No Hit
CTCGGTTGATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGG4000.1286674236600092No Hit
TCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAA3970.12770241798255913No Hit
GTCGACTCACCCTGCCCCGATTAACGTTGGACAGGAACCCTTGGTCTTCC3970.12770241798255913No Hit
CCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGGAGCCCAGAGCCTG3920.126094075186809No Hit
CCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGG3910.12577240662765898No Hit
CCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCA3860.12416406383190888No Hit
CACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAA3840.12352072671360884No Hit
GGCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCT3800.12223405247700873No Hit
ATCAAATCGTACCCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAG3800.12223405247700873No Hit
CCCAGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGGAAACGATG3800.12223405247700873No Hit
GCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGA3780.12159071535870869No Hit
GTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCA3780.12159071535870869No Hit
GCGACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCATCAGGTAACATC3760.12094737824040863No Hit
GAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGG3710.11933903544465853No Hit
CTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACC3710.11933903544465853No Hit
CGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGG3710.11933903544465853No Hit
GCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTC3680.11837402976720847No Hit
GTGAGGGTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGA3680.11837402976720847No Hit
AAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGA3670.11805236120805845No Hit
TCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCAT3650.1174090240897584No Hit
TGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAA3620.11644401841230832No Hit
CGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAAT3590.11547901273485826No Hit
GTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAA3570.1148356756165582No Hit
ATGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTG3560.11451400705740819No Hit
CTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTT3490.11226232714335804No Hit
GCCAGCTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCAC3460.11129732146590796No Hit
CAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATG3400.10936731011100782No Hit
CCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCA3370.10840230443355775No Hit
GTGAAATTGAACTCGCTGTGAAGATGCAGTGTACCCGCGGCAAGACGGAA3320.10679396163780763No Hit
TGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTG3320.10679396163780763No Hit
TCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGC3270.10518561884205753No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA3250.10454228172375747TruSeq Adapter, Index 2 (97% over 37bp)
GTTGATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTCG3240.10422061316460744No Hit
CCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGA3220.10357727604630741No Hit
CTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGTAC3220.10357727604630741No Hit
CCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTA3190.10261227036885733No Hit
GGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGAT3160.10164726469140727No Hit
GCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAG3110.10003892189565716No Hit
CTCAGTTCTCCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCACGACG3110.10003892189565716No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTTA608.013376E-872.55
GCGACAA1102.5465852E-1159.3181841
TTGAACG753.7527207E-758.0000047
GTAGGGG2750.058.01
CCCACGT858.9047535E-751.1764683
GGGGAGC8500.049.470591
TGAACGC1056.885966E-848.3333328
CGACAAA1203.607056E-948.3333322
GCGTACA1551.4551915E-1146.7741932
GGGAGCG9000.046.722222
AGCGTAC1453.8198777E-1045.01
GGCGTAA657.3287537E-444.6153831
CGGAGTA1002.7280348E-643.54
TCGTCAT4050.042.9629632
CGTTAGA2550.042.6470577
CGTCATC4500.041.8888853
GACAAAT3650.041.7123263
ATCACGC4600.040.9782647
ACAAATG3750.040.6000024
GTGACAA2400.039.2708321
Produced by FastQC (version 0.11.2)