FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n01_62o2.3510000009e988.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n01_62o2.3510000009e988.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226542
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT53742.3721870558218785TruSeq Adapter, Index 27 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGTAC451.19187316E-464.444443
CGGGGAT400.006044869354.3751
GCGAGGA400.006044869354.3752
CGGGGGG950.004719661530.5263141
CCGGAGA1100.00965610226.3636381
GGGGGGC1409.970674E-425.8928572
CCGGGTG1550.00180419323.3870961
GGTGCAA1902.1859015E-422.8947355
GGGGAAT3157.2307375E-720.7142871
ACTACAC1950.00682462418.5897455
CCGGGCA2050.00910525317.6829261
CCTAAAA2050.00910525317.6829267
CCAACGC602.4172262E-416.916668110-114
CTTTTAT2700.002323917316.111111
TTCAAAT3406.238693E-414.926476
GGAAAAT3000.00468716214.51
GGTTTCA6954.1853127E-813.56115154
GGAAGAT3850.001596483413.1818191
GAGCACA72650.012.2746059
AGAGCAC73850.012.0751528
Produced by FastQC (version 0.11.2)