FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n01_62o1.3510000009e97b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n01_62o1.3510000009e97b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375611
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT47401.2619438727832784TruSeq Adapter, Index 7 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAC450.00963448848.3333321
CGGAGTT951.0276654E-438.1578941
CCGGTGG900.0036253732.222223
GATCGCG1000.006069694629.05
TAAGGTT1550.001806396623.3870964
CGGGTTA1800.0043021520.138891
ATCCAAC1850.00504112219.5945956
ACATCTA2256.856752E-419.3333344
GTTGGAT1950.006832795718.5897451
CGGGAAG2550.00158849517.0588231
GGGGAAT5753.4597178E-916.3913061
CTCCAAT4201.9643469E-413.8095241
GCAGAAT3150.006488344613.8095241
AGATTGA3700.00118563613.7162174
GAACCCG3950.001940425312.8481024
GGGAAAG6855.196471E-712.700731
TCTTGGC5351.1017419E-412.1962627
AATATCC4500.0051494511.2777793
CCGGAAT2650.00885854410.9433961
GTTTTGC4800.00832053910.5729175
Produced by FastQC (version 0.11.2)