FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n01_62n2.3510000009e839.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n01_62n2.3510000009e839.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384398
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT216995.644930514726924TruSeq Adapter, Index 7 (97% over 38bp)
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC8790.2286692438566278No Hit
GGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGG6050.15738895623806576No Hit
CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA4520.11758645986711691No Hit
CCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG4380.11394440137565752No Hit
GGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTA4010.1043189610768006No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATATCGTAT4010.1043189610768006TruSeq Adapter, Index 7 (97% over 38bp)
GGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGT3930.10223778479596668No Hit
GCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCA3900.10145734369065396No Hit
CCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGA3860.10041675555023698No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAATCG1652.7284841E-1143.9393923
TCGTACC1652.7284841E-1143.9393927
ATCGTAC1807.094059E-1140.277786
CGTACCC1901.2914825E-1038.1578948
ACCCGCT4100.037.134148145
CGGAGTA2154.838512E-1033.7209324
GGAGTAC2455.4569682E-1132.551025
GAGTACC2051.1346856E-831.8292686
AGTACCT2101.4350007E-831.0714287
CCGGAGT2401.5752448E-930.2083343
CCATACG1453.4848646E-530.06
GTAGGGG1952.6002454E-729.7435871
TGCGACA1000.006069868329.09
CCGACTA1000.006069868329.05
TGTCCGA1000.006069868329.08
GGGAGCG3800.028.6184222
CGAACCG2553.0104275E-928.4313723
TCGTCAT1555.4917415E-528.0645162
TAGGGGA2354.2831743E-827.7659592
CGGCCTA1050.007702645427.6190471
Produced by FastQC (version 0.11.2)