FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n01_62n1.3510000009e82c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n01_62n1.3510000009e82c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345755
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT73792.134170149383234TruSeq Adapter, Index 2 (97% over 37bp)
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC16410.4746135269193504No Hit
GGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGG16190.46825063990397825No Hit
GCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCA14690.4248673193446227No Hit
GGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTA11700.3383899003629738No Hit
GGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGT11660.3372330118147243No Hit
CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA11270.32595334846929186No Hit
CCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG9960.2880652485141213No Hit
GTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGA9450.2733149195239403No Hit
CCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGA8230.23802981880233112No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA7700.2227010455380255No Hit
GTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGG7360.21286749287790485No Hit
CCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCC7340.21228904860378012No Hit
ATCAAATCGTACCCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAG6720.19435727610591313No Hit
CCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC6580.19030816618703994No Hit
CTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCACCTACA6290.1819207242122312No Hit
CTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGT6240.18047461352691935No Hit
GGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAG6030.17440094864860956No Hit
GGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCA5840.16890572804442452No Hit
CTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCG5740.16601350667380083No Hit
TCAAATCGTACCCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAGG5430.15704762042486733No Hit
CCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAAC5420.15675839828780497No Hit
CTCGTCATCACGCCTCAGCCTTGATTTTCCGGATTTGCCTGGAAAACCAG5360.1550230654654307No Hit
GGCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCT5200.15039551127243278No Hit
CCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTCACCGACGC5200.15039551127243278No Hit
CCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATTCAGAACCA4970.14374340211999825No Hit
CTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGG4930.14258651357174879No Hit
CCCGTTACATCTTCCGCGCAGGCCGACTCGACCAGTGAGCTATTACGCTT4880.14114040288643692No Hit
TGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGT4850.1402727364752498No Hit
CCCCGTTACATCTTCCGCGCAGGCCGACTCGACCAGTGAGCTATTACGCT4840.13998351433818745No Hit
CCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTA4770.13795895937875083No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC4740.13709129296756375No Hit
CCTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATG4720.136512848693439No Hit
GCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTC4560.13188529450044106No Hit
CCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTCACCGACGCT4340.1255224074850689No Hit
CCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGT4300.12436551893681942No Hit
CTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGTCAGCAT4280.12378707466269467No Hit
GGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGAT4250.12291940825150757No Hit
GAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTAT4230.12234096397738282No Hit
GCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACC3980.11511041055082356No Hit
CCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAG3900.1127966334543246No Hit
CCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAG3880.11221818918019985No Hit
GCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGG3860.11163974490607512No Hit
GGTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAG3860.11163974490607512No Hit
GTCGACTCACCCTGCCCCGATTAACGTTGGACAGGAACCCTTGGTCTTCC3690.10672296857601482No Hit
GCCAGCTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCAC3600.10411996934245346No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGG2050.074.2682951
CGTACCT953.1313903E-853.4210474
CGGAGTA1550.051.451614
TATAGCG450.00963384748.3333329
GAACCGG3800.045.789474
GAGTACC1751.8189894E-1245.571436
GCGAACC3900.044.6153872
GCCGGTC657.3299004E-444.6153832
GGAGTAC1801.8189894E-1244.3055535
TGCGTAC1201.9684376E-742.2916682
CGGGGGA4150.041.927718
TAGGGGA3850.041.4285742
CCGGGAA1401.4098987E-841.428571
CATCACG2650.041.037746
GGGAGCG8400.040.5654751
ACCGGGG4400.039.5454566
GGGGAGC7550.039.370861
ATCACGC2800.038.8392877
CGAACCG4950.038.0808073
AGTACCT2109.094947E-1237.976197
Produced by FastQC (version 0.11.2)