FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n01_61o2.3510000009e9a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n01_61o2.3510000009e9a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617708
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT52760.8541252501181787TruSeq Adapter, Index 2 (97% over 37bp)
GCTAAATACTCCCTAGTGACCGATAGTGAACCAGTACCGTGAGGGAAAGG31810.5149682374196222No Hit
GAGCGTTCTAAGGGCGTTGAAGGTCGATCGTGAGGACGGCTGGAGCGCTT23550.3812480978067307No Hit
GGAGAGCGTTCTAAGGGCGTTGAAGGTCGATCGTGAGGACGGCTGGAGCG22910.3708872153185648No Hit
CTCGGCTTAGGTCCCGACTAACCCTGGGCGGACGAGCCTTCCCCAGGAAA21660.3506511167088657No Hit
CTCCATTGCTCAAACAAAAACAACTGGTACAGGAATATCAACCTGTTGTC20660.3344622378211064No Hit
GGCGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACTAA18850.3051603670342622No Hit
CTCGCTTTCGCTGCGGCTCCGTCTCTTCAACTTAACCTCGCATGCAATCG16670.26986861105894694No Hit
CTCGCTTTCGCTGCGGCTCCGTCTCTTCGACTTAACCTCGCATGCAATCG16620.26905916711455896No Hit
GCGGGTTAGAGGGTTCATAACACAAGGGTAGTATCCCACCAGCGCCTCCT15970.2585363958375155No Hit
CGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACTAACA15840.25643184158210675No Hit
GCGTTCTAAGGGCGTTGAAGGTCGATCGTGAGGACGGCTGGAGCGCTTAG14840.24024296269434747No Hit
CCTCCATTGCTCAAACAAAAACAACTGGTACAGGAATATCAACCTGTTGT14620.23668140933904044No Hit
CGTCCCTCCATTGCTCAAACAAAAACAACTGGTACAGGAATATCAACCTG14360.23247230082822304No Hit
GTCGATCGTGAGGACGGCTGGAGCGCTTAGAAGTGAGAATGCCGGTATGA13120.21239809100740153No Hit
CTAAATACTCCCTAGTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGT12090.19572354575300954No Hit
GTAGGAGAGCGTTCTAAGGGCGTTGAAGGTCGATCGTGAGGACGGCTGGA12030.19475221301974396No Hit
CCCCATTCGGAAATCTCTGGATCATAGCTTACTTACAGCTCCCCAAAGCA11830.1915144372421921No Hit
GTGAAAAGCACCCCGGAAGGGGAGTGAAATAGATCCTGAAACCGTGTGCC11810.19119065966443693No Hit
GCCGGTATGAGTAGCGAAAGACAGGTGAGAATCCTGTCCACCGTATGACT11470.18568644084259878No Hit
GGGCGTTGAAGGTCGATCGTGAGGACGGCTGGAGCGCTTAGAAGTGAGAA11110.17985844444300544No Hit
CCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCC11040.1787252229208623No Hit
CCCTAGTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGTGAAAAGCAC10800.17483989198780006No Hit
CGATAGTGAACCAGTACCGTGAGGGAAAGGTGAAAAGCACCCCGGAAGGG10790.17467800319892246No Hit
GGGAAACAGCCCAGACCACCAGCTAAGGTCCCAAAATATATGTTAAGTGG10150.16431712071075655No Hit
GTTCTAAGGGCGTTGAAGGTCGATCGTGAGGACGGCTGGAGCGCTTAGAA9500.153794349433713No Hit
CGCCGGTTCATTCTACAAAAGGCACGCCATCACTCATTAACGAGCTTTGA9060.14667124272309892No Hit
CCTCGACCGATTAGTATTGGTCCGCTCCAACTATCACTAGCCTTCCACTT8970.1452142436232006No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACTAACACCGAT8910.14424291088993504No Hit
GTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGTGAAAAGCACCCCGG8400.13598658265717783No Hit
CCCATTCGGAAATCTCTGGATCATAGCTTACTTACAGCTCCCCAAAGCAT8370.13550091629054506No Hit
CAAACACTCAATATCAAACTACAGTAAAGCTCCATGGGGTCTTTCCGTCC8030.1299966974687069No Hit
GCCGGTTCATTCTACAAAAGGCACGCCATCACTCATTAACGAGCTTTGAC7990.12934914231319652No Hit
CGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTCGGTCCCTATCCGTCGC7900.1278921432132982No Hit
GGCCTATTCACTGCGGCTGACCGAAGTCAGCACCCCTTCTCCCGAAGTTA7750.12546381138013432No Hit
CCGATAGTGAACCAGTACCGTGAGGGAAAGGTGAAAAGCACCCCGGAAGG7660.12400681228023597No Hit
CCGGTTCATTCTACAAAAGGCACGCCATCACTCATTAACGAGCTTTGACT7490.12125470286931689No Hit
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG7440.12044525892492892No Hit
CCCGACTAACCCTGGGCGGACGAGCCTTCCCCAGGAAACCTTAGTTATAC7410.11995959255829615No Hit
TGAAGGTCGATCGTGAGGACGGCTGGAGCGCTTAGAAGTGAGAATGCCGG7120.11526481768084597No Hit
CGAAAGGGAAACAGCCCAGACCACCAGCTAAGGTCCCAAAATATATGTTA6990.11316026342543725No Hit
GTGAACCAGTACCGTGAGGGAAAGGTGAAAAGCACCCCGGAAGGGGAGTG6980.11299837463655966No Hit
CCCCGTCACAACTTGTCCTTAAAGAGTAAAGCATTTAACTCTACTCAAGA6900.11170326432553893No Hit
GCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACTAACAC6800.110084376436763No Hit
CTCAAACAAAAACAACTGGTACAGGAATATCAACCTGTTGTCCATCGCCT6680.1081417109702319No Hit
CCCGTCACAACTTGTCCTTAAAGAGTAAAGCATTTAACTCTACTCAAGAC6640.1074941558147215No Hit
TAGGAGAGCGTTCTAAGGGCGTTGAAGGTCGATCGTGAGGACGGCTGGAG6610.10700848944808873No Hit
GCCGGTATGAGTAGCGAAAGACAGGTGAGAATCCTGTCCACCGTATAACT6610.10700848944808873No Hit
CTGAAAAGTAATATCCTATAAAAGATATTGGGTTCCCCCATTCGGAAATC6580.10652282308145596No Hit
GGTCGATCGTGAGGACGGCTGGAGCGCTTAGAAGTGAGAATGCCGGTATG6440.10425638003716967No Hit
GCCCAGACCACCAGCTAAGGTCCCAAAATATATGTTAAGTGGAAAAGGAT6430.10409449124829206No Hit
GTCCCGACTAACCCTGGGCGGACGAGCCTTCCCCAGGAAACCTTAGTCAT6430.10409449124829206No Hit
CCCGACTAACCCTGGGCGGACGAGCCTTCCCCAGGAAACCTTAGTCATAC6410.10377071367053689No Hit
CTGGGCTACACACGTGCTACAATGGGAAGTACAACGAGTCGCTAGACCGC6400.10360882488165929No Hit
GAAAGGCGTAACGATTTGGGCACTGTCTCAACGAGAGACTCGGTGAAATT6380.10328504730390412No Hit
CCCCCATTCGGAAATCTCTGGATCATAGCTTACTTACAGCTCCCCAAAGC6210.10053293789298504No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACC2300.078.8043442
TCGACCG2400.075.5208363
GGCGCAC4350.063.3333361
GCGCACG4950.057.1212122
CGCACGG5100.054.019613
TAGAACG2700.053.7037145
CTCGCTT11100.049.639641
TCGCTGC11000.049.431828
CGCTGCG11900.045.693289
AGAGCGT7050.045.2482263
TTCGCTG12650.044.1304367
TCGCTTT12550.042.7490042
TCTCGCG1201.9718027E-742.2916686
CGGCTAC1401.412991E-841.4285741
GAGAGCG7800.040.8974382
CCGATTA4450.040.730347
GCACGGT6900.039.9275364
ACCGATT4600.039.4021726
GGTTCGG2400.039.2708324
GGAGAGC8200.038.902441
Produced by FastQC (version 0.11.2)