FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n01_61n2.3510000009e901.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n01_61n2.3510000009e901.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280591
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT231618.254363112145436TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT9810.34961919662426805TruSeq Adapter, Index 27 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA6780.24163283925713938TruSeq Adapter, Index 27 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGA604.938959E-448.3333324
TCGGTTG850.0027386434.117652
GAGCACA107800.033.358079
AGAGCAC109550.032.8913738
AAGAGCA111000.032.461717
GAAGAGC112550.031.9502456
TCGGAAG116050.030.924173
GGAAGAG116800.030.7256015
CGGAAGA117950.030.5489644
ATCGGAA118250.030.3488372
CTCGCTT1204.0506807E-430.2083341
GATCGGA119250.030.1551341
CGCACGG1000.006067111629.03
GGCGCAC1100.00965995926.3636361
CGTTACA1409.976624E-425.8928592
GAGCGTT1600.002171278222.656258
GGGAACG1600.002171278222.656253
CTCGGTT2053.6573716E-421.2195131
GTTAGAA1750.00365226620.7142875
GCACGGT1850.005038003419.5945954
Produced by FastQC (version 0.11.2)