FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n01_61n1.3510000009e846.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n01_61n1.3510000009e846.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396055
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT395769.9925515395589TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT16490.4163563141482875TruSeq Adapter, Index 27 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA8400.21209175493302695TruSeq Adapter, Index 27 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG4530.11437805355316812TruSeq Adapter, Index 27 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGAT502.0087557E-458.01
CGAGGGT450.00963486948.3333361
CCCGGTA752.5477695E-548.3333362
GCGTTCT1601.0077201E-940.781253
GAGCGTT1651.3606041E-939.5454561
CCGGTAC1001.3899329E-436.253
CGGTACA1051.8517005E-434.5238084
CGTTCTA1905.4096745E-934.3421024
CCGCACG1301.6522696E-533.4615369
CGCACGG1352.138957E-532.222223
AGCGTTC2303.4764525E-828.3695662
GAGCACA223600.028.014319
AGAGCAC226100.027.7045548
CTCGTTT1050.00770292427.6190471
AAGAGCA228600.027.4650027
GAAGAGC230850.027.1973156
GCCGGTT1358.0740516E-426.851852
CCGGTTC1358.0740516E-426.851853
CGGAAGA234550.026.644644
GGAAGAG236950.026.5583465
Produced by FastQC (version 0.11.2)