FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n01_59n2.3510000009e8cc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n01_59n2.3510000009e8cc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258319
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT247989.59975843821012TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT9800.37937588795249283TruSeq Adapter, Index 27 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA7890.305436301627058TruSeq Adapter, Index 27 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA100900.041.531229
AGAGCAC102200.040.9319958
AAGAGCA104100.040.4634977
GAAGAGC105600.039.888736
TCGGAAG107850.039.056563
GGAAGAG109350.038.6534045
CGGAAGA109850.038.4114724
ATCGGAA110050.038.2757842
GATCGGA110850.037.9995461
TAGGGCG1000.006066231529.0145
GCGGGAT1050.00769804427.6190471
GAGATTC1650.002595689421.9696962
CTTGAAA39250.021.09426760-64
TTGAAAA41000.020.93658660-64
GCTTGAA40200.020.66791260-64
TGCTTGA41050.020.23995260-64
TTAAACC2155.042191E-420.2325572
CATGAAC1800.00429857320.1388871
TCTGCTT42150.019.9181555-59
CTGCTTG41550.019.54271955-59
Produced by FastQC (version 0.11.2)