FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n01_59n1.3510000009e802.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n01_59n1.3510000009e802.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258751
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT140205.418336547491604TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT5320.20560307013306228TruSeq Adapter, Index 27 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA4750.18357416976166274TruSeq Adapter, Index 27 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGCC1001.3886011E-436.251
CTAGCCT900.003623301232.222222
CGGATGT1050.00769806927.6190473
TTGTACG1358.066443E-426.8518529
CGGCACG1658.394921E-526.3636366
GGAGCGT1500.001491287324.1666682
GAGCACA106900.021.8381679
AGAGCAC108950.021.3607148
AAGAGCA111950.020.853067
CTTGTAC1750.001077422520.7142878
GCTTGTA1750.003651509520.7142877
GGGAGCG1750.003651509520.7142871
GAAGAGC112950.020.6684366
TCGGAAG114550.020.1898733
CCGACTT1800.004298592520.138894
GGAAGAG116300.020.0730865
ATCGGAA116950.019.7755452
CGGAAGA117350.019.7081384
GATCGGA117800.019.6328531
GGCCAAT1850.005036963619.5945951
Produced by FastQC (version 0.11.2)