FastQCFastQC Report
Thu 27 Jul 2017
000000000-B5WD2_l01n02_vgt2_n.352000000987f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B5WD2_l01n02_vgt2_n.352000000987f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1510318
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT288271.9086708891769812Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCCCTATGTGTAGATCT40490.26808923683621594Illumina Single End PCR Primer 1 (96% over 32bp)
CGCATTTTTAAATGGCGTACATGCCTCTTTTCATTCGGTAAAGAGGACTT22950.1519547538995099No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC21160.14010294520756555No Hit
CCCAATAGTGCCGTTGCAGCTTTCCTTTGTTTTGAACCCGCTCTCAGCAG20780.13758691878134274No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT19750.13076716294184404No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG17950.11884914302815698No Hit
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG16380.10845398121455216No Hit
GCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCC15860.10501099768393145No Hit
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGA15250.10097211315762641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGTC41850.065.98566452
GTGTAGA48850.065.1995941
TAGATCT45950.065.1251444
TGGTCGC41100.064.8053554
TGTAGAT48800.064.6926242
AGATCTC45450.064.14741545
GTCGCCG42600.063.83802856
ATCTCGG45600.063.78289847
TCTCGGT43950.063.31057748
GTGCCTC40400.063.06930531
ATGTGTA51500.062.93203739
TCTATGT40650.062.50922836
CCTCTAT40550.062.2318134
AGTGTGC48600.062.15020428
CTCTATG41300.061.61017235
GAGTGTG49650.061.54078727
GTGGTCG44550.061.43659253
TGTGTAG52450.061.391840
CGGTGGT46250.060.9189251
GGTCGCC44600.060.58296255