Basic Statistics
Measure | Value |
---|---|
Filename | 000000000-B5WD2_l01n02_vgt2_n.352000000987f4.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1510318 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 47 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT | 28827 | 1.9086708891769812 | Illumina Single End PCR Primer 1 (96% over 32bp) |
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCCCTATGTGTAGATCT | 4049 | 0.26808923683621594 | Illumina Single End PCR Primer 1 (96% over 32bp) |
CGCATTTTTAAATGGCGTACATGCCTCTTTTCATTCGGTAAAGAGGACTT | 2295 | 0.1519547538995099 | No Hit |
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC | 2116 | 0.14010294520756555 | No Hit |
CCCAATAGTGCCGTTGCAGCTTTCCTTTGTTTTGAACCCGCTCTCAGCAG | 2078 | 0.13758691878134274 | No Hit |
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT | 1975 | 0.13076716294184404 | No Hit |
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG | 1795 | 0.11884914302815698 | No Hit |
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG | 1638 | 0.10845398121455216 | No Hit |
GCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCC | 1586 | 0.10501099768393145 | No Hit |
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGA | 1525 | 0.10097211315762641 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GGTGGTC | 4185 | 0.0 | 65.985664 | 52 |
GTGTAGA | 4885 | 0.0 | 65.19959 | 41 |
TAGATCT | 4595 | 0.0 | 65.12514 | 44 |
TGGTCGC | 4110 | 0.0 | 64.80535 | 54 |
TGTAGAT | 4880 | 0.0 | 64.69262 | 42 |
AGATCTC | 4545 | 0.0 | 64.147415 | 45 |
GTCGCCG | 4260 | 0.0 | 63.838028 | 56 |
ATCTCGG | 4560 | 0.0 | 63.782898 | 47 |
TCTCGGT | 4395 | 0.0 | 63.310577 | 48 |
GTGCCTC | 4040 | 0.0 | 63.069305 | 31 |
ATGTGTA | 5150 | 0.0 | 62.932037 | 39 |
TCTATGT | 4065 | 0.0 | 62.509228 | 36 |
CCTCTAT | 4055 | 0.0 | 62.23181 | 34 |
AGTGTGC | 4860 | 0.0 | 62.150204 | 28 |
CTCTATG | 4130 | 0.0 | 61.610172 | 35 |
GAGTGTG | 4965 | 0.0 | 61.540787 | 27 |
GTGGTCG | 4455 | 0.0 | 61.436592 | 53 |
TGTGTAG | 5245 | 0.0 | 61.3918 | 40 |
CGGTGGT | 4625 | 0.0 | 60.91892 | 51 |
GGTCGCC | 4460 | 0.0 | 60.582962 | 55 |