Basic Statistics
Measure | Value |
---|---|
Filename | 000000000-B5WD2_l01n02_vgt2_b.35200000098797.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1785326 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 47 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT | 4111 | 0.2302660690540551 | Illumina Single End PCR Primer 1 (96% over 32bp) |
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC | 2865 | 0.16047489366087764 | No Hit |
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG | 2663 | 0.14916043344464822 | No Hit |
CGCATTTTTAAATGGCGTACATGCCTCTTTTCATTCGGTAAAGAGGACTT | 2417 | 0.13538143733973515 | No Hit |
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGA | 2155 | 0.12070624636620986 | No Hit |
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT | 2154 | 0.1206502341869216 | No Hit |
CGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACA | 1990 | 0.11146423678364624 | No Hit |
CCCAATAGTGCCGTTGCAGCTTTCCTTTGTTTTGAACCCGCTCTCAGCAG | 1965 | 0.11006393230143963 | No Hit |
CACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCC | 1864 | 0.10440670219332492 | No Hit |
CGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGAT | 1832 | 0.10261431245610045 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GGTGGTC | 725 | 0.0 | 57.931034 | 52 |
GTGTAGA | 1015 | 0.0 | 55.86207 | 41 |
TAGATCT | 1015 | 0.0 | 51.724136 | 44 |
AGATCTC | 990 | 0.0 | 51.262627 | 45 |
TGTAGAT | 1100 | 0.0 | 50.272724 | 42 |
ATCTCGG | 1095 | 0.0 | 47.30594 | 47 |
TCTCGGT | 1030 | 0.0 | 45.873783 | 48 |
GTGCCTC | 860 | 0.0 | 45.581394 | 31 |
TCTATGT | 905 | 0.0 | 44.86188 | 36 |
TGGTCGC | 890 | 0.0 | 44.83146 | 54 |
GTCGCCG | 925 | 0.0 | 43.891888 | 56 |
ATGTGTA | 1280 | 0.0 | 43.75 | 39 |
GAGTGTG | 1180 | 0.0 | 43.601696 | 27 |
AGTGTGC | 1205 | 0.0 | 41.825726 | 28 |
CCTCTAT | 1005 | 0.0 | 39.701492 | 34 |
CCCTATG | 205 | 0.0 | 37.56097 | 35 |
GTGCCCC | 235 | 0.0 | 37.23404 | 31 |
GTGGTCG | 1150 | 0.0 | 37.130432 | 53 |
CTCTATG | 1075 | 0.0 | 37.11628 | 35 |
TGTGTAG | 1540 | 0.0 | 36.81818 | 40 |