FastQCFastQC Report
Thu 27 Jul 2017
000000000-B5WD2_l01n02_vgt2_b.35200000098797.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B5WD2_l01n02_vgt2_b.35200000098797.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1785326
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT41110.2302660690540551Illumina Single End PCR Primer 1 (96% over 32bp)
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC28650.16047489366087764No Hit
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG26630.14916043344464822No Hit
CGCATTTTTAAATGGCGTACATGCCTCTTTTCATTCGGTAAAGAGGACTT24170.13538143733973515No Hit
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGA21550.12070624636620986No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT21540.1206502341869216No Hit
CGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACA19900.11146423678364624No Hit
CCCAATAGTGCCGTTGCAGCTTTCCTTTGTTTTGAACCCGCTCTCAGCAG19650.11006393230143963No Hit
CACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCC18640.10440670219332492No Hit
CGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGAT18320.10261431245610045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGTC7250.057.93103452
GTGTAGA10150.055.8620741
TAGATCT10150.051.72413644
AGATCTC9900.051.26262745
TGTAGAT11000.050.27272442
ATCTCGG10950.047.3059447
TCTCGGT10300.045.87378348
GTGCCTC8600.045.58139431
TCTATGT9050.044.8618836
TGGTCGC8900.044.8314654
GTCGCCG9250.043.89188856
ATGTGTA12800.043.7539
GAGTGTG11800.043.60169627
AGTGTGC12050.041.82572628
CCTCTAT10050.039.70149234
CCCTATG2050.037.5609735
GTGCCCC2350.037.2340431
GTGGTCG11500.037.13043253
CTCTATG10750.037.1162835
TGTGTAG15400.036.8181840