Basic Statistics
Measure | Value |
---|---|
Filename | 000000000-B5WD2_l01n02_vgt1_n.352000000987e7.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 2609584 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT | 12370 | 0.4740219130712021 | Illumina Single End PCR Primer 1 (96% over 32bp) |
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG | 3764 | 0.14423754897332294 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GGTGGTC | 1890 | 0.0 | 66.296295 | 52 |
GTGCCTC | 1975 | 0.0 | 60.962025 | 31 |
GAGTGTG | 2305 | 0.0 | 59.826466 | 27 |
AGTGTGC | 2300 | 0.0 | 59.347824 | 28 |
GTGTAGA | 2385 | 0.0 | 59.14046 | 41 |
TAGATCT | 2295 | 0.0 | 57.494553 | 44 |
AGATCTC | 2250 | 0.0 | 57.08889 | 45 |
TGGTCGC | 2055 | 0.0 | 57.05596 | 54 |
TCTATGT | 2105 | 0.0 | 56.199528 | 36 |
TGTAGGG | 2680 | 0.0 | 55.895523 | 17 |
CTCTATG | 2140 | 0.0 | 55.77103 | 35 |
ATCTCGG | 2305 | 0.0 | 55.574837 | 47 |
GTCGCCG | 2125 | 0.0 | 55.341175 | 56 |
TGTAGAT | 2545 | 0.0 | 55.009827 | 42 |
ATGTGTA | 2715 | 0.0 | 54.014732 | 39 |
TCTCGGT | 2355 | 0.0 | 53.949043 | 48 |
GTCGTGT | 2755 | 0.0 | 52.59528 | 13 |
TCGTGTA | 2750 | 0.0 | 52.436363 | 14 |
CCTCTAT | 2300 | 0.0 | 51.282608 | 34 |
GTGTAGG | 2920 | 0.0 | 51.181507 | 16 |