FastQCFastQC Report
Thu 27 Jul 2017
000000000-B5WD2_l01n02_vgt1_n.352000000987e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B5WD2_l01n02_vgt1_n.352000000987e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2609584
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT123700.4740219130712021Illumina Single End PCR Primer 1 (96% over 32bp)
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG37640.14423754897332294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGTC18900.066.29629552
GTGCCTC19750.060.96202531
GAGTGTG23050.059.82646627
AGTGTGC23000.059.34782428
GTGTAGA23850.059.1404641
TAGATCT22950.057.49455344
AGATCTC22500.057.0888945
TGGTCGC20550.057.0559654
TCTATGT21050.056.19952836
TGTAGGG26800.055.89552317
CTCTATG21400.055.7710335
ATCTCGG23050.055.57483747
GTCGCCG21250.055.34117556
TGTAGAT25450.055.00982742
ATGTGTA27150.054.01473239
TCTCGGT23550.053.94904348
GTCGTGT27550.052.5952813
TCGTGTA27500.052.43636314
CCTCTAT23000.051.28260834
GTGTAGG29200.051.18150716