FastQCFastQC Report
Thu 27 Jul 2017
000000000-B5WD2_l01n02_vgt1_b.3520000009878a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B5WD2_l01n02_vgt1_b.3520000009878a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2226463
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT22310.10020377612383409No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGTC3550.047.32394452
GAGTGTG6550.036.87022827
AGTGTGC6550.035.80152528
GTGTAGA6950.034.24460241
GTGCCTC4950.033.23232331
TGTAGGG7800.032.30769317
AGATCTC6200.031.04838845
TAGATCT6300.030.55555344
GATTAGT5250.030.09
ATCTCGG6700.029.25373347
TCTCGGT6700.029.25373348
GTCGTGT8250.028.42424413
GTGTAGG9200.028.15217616
ATGTGTA9150.027.92349839
TGTAGAT8150.027.91410842
TCTATGT6700.027.68656736
TCGTGTA8400.027.49999814
TGGTCGC6150.026.74796954
CGGTGGT6950.025.68345351
CGATTAG6050.025.4545448
Produced by FastQC (version 0.11.2)