FastQCFastQC Report
Thu 27 Jul 2017
000000000-B5WD2_l01n02_oat2_n.352000000987da.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B5WD2_l01n02_oat2_n.352000000987da.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1385834
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT170021.2268424645375997Illumina Single End PCR Primer 1 (96% over 32bp)
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT33600.24245328084027382No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCCCTATGTGTAGATCT25170.18162348448659796Illumina Single End PCR Primer 1 (96% over 32bp)
GGCAGTTTGACTGGGGCGGTCGCCTCCTAAAAGGTAACGGAGGCGCCCAA15150.1093204525217306No Hit
CCGGGATGGACGCACCGCTGGTGTACCAGTTGTTCTGCCAAGGGCATCGC15050.10859886537637263No Hit
GCCGGTATGAGTAGCGAAAGAGGGGTGAGAATCCCCTCCACCGAATGCCT14820.10693921494204933No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC14820.10693921494204933No Hit
CGCATTTTTAAATGGCGTACATGCCTCTTTTCATTCGGTAAAGAGGACTT14780.10665058008390614No Hit
GTCAGCTCCATGTGTCGCCACACTTCCACCTCAGACCTATCAACCTGATC14510.10470229479143967No Hit
CCCAATAGTGCCGTTGCAGCTTTCCTTTGTTTTGAACCCGCTCTCAGCAG14260.10289832692804478No Hit
GCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCC14030.10123867649372148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGTC26700.067.3782752
CTCTATG25400.066.27952635
AGTGTGC31650.066.0189628
GAGTGTG32450.065.7935327
TGGTCGC26200.065.7251954
GTGCCTC26200.065.7251931
TCTCGGT28500.065.5789448
GTGTAGA34150.065.4904841
GTGTAGG34650.064.949516
TGTAGAT33750.064.91852642
AGATCTC29850.064.84086645
GTCGCCG27150.064.32780556
ATGTGTA35450.064.2736239
CCTCTAT26300.063.74524334
GTCGTGT34600.063.5260113
ATCTCGG30550.063.3551647
TAGATCT31500.063.2222244
TCGTGTA34500.063.20289614
GCGTCGT36150.062.7385911
TCGCCGT25300.062.1146257
Produced by FastQC (version 0.11.2)