FastQCFastQC Report
Thu 27 Jul 2017
000000000-B5WD2_l01n02_oat2_b.3520000009877d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B5WD2_l01n02_oat2_b.3520000009877d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1374789
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT100350.7299301929241505Illumina Single End PCR Primer 1 (96% over 32bp)
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT30280.22025198048573272No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC17860.12991084450050153No Hit
GGCAGTTTGACTGGGGCGGTCGCCTCCTAAAAGGTAACGGAGGCGCCCAA14710.10699823754772551No Hit
GTCAGCTCCATGTGTCGCCACACTTCCACCTCAGACCTATCAACCTGATC13810.10045177841836092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGTC13700.066.6788352
GTGTAGG16500.063.84848416
GTGCCTC12750.063.68627531
GAGTGTG15350.063.6156327
AGTGTGC15250.063.5737728
CTCTATG13150.063.3460135
TGGTCGC13650.063.33333254
TGTAGAT16250.062.24615542
GTCGTGT16950.061.3274413
TCGTGTA16750.061.22387714
GTGTAGA16700.061.19760541
ATGTGTA17200.060.63953439
AGATCTC15600.060.12820445
TCTCGGT15200.059.86842348
CCTCTAT13750.059.81818434
GTCGCCG14750.059.32203356
TGTAGGG17950.058.4958217
TAGATCT16650.058.43843544
GCGTCGT17900.057.8770911
ATCTCGG16300.057.7607447
Produced by FastQC (version 0.11.2)