FastQCFastQC Report
Thu 27 Jul 2017
000000000-B5WD2_l01n02_oat1_n.352000000987cd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B5WD2_l01n02_oat1_n.352000000987cd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2713401
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT205050.755693684788942Illumina Single End PCR Primer 1 (96% over 32bp)
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG86200.31768249514170593No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT82630.30452557509929423No Hit
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGA66090.24356886431456315No Hit
GCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAAGGCGCAACGAT58670.21622310893229568No Hit
GCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCT53650.19772234181383436No Hit
CGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACA50770.18710835589726693No Hit
GCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACAC47810.17619953703857263No Hit
CACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT46970.17310379114624047No Hit
TTCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCC45390.1672808405392347No Hit
GCGAAATATAGTAGCGAAGTTCCTGATTCCACACTGCCAAGAAAAGCCTC42380.1561877510917111No Hit
CCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCA41320.15228121460853003No Hit
CCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCATTC40060.14763759577003177No Hit
GTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAA37800.1393085651549476No Hit
GTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACC37370.13772383809101568No Hit
CACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCC37160.13694990161793263No Hit
GGCGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAA36870.13588113220272272No Hit
CCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACC36860.13584427808495683No Hit
GTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAG36790.13558629926059584No Hit
CCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGA36760.13547573690729825No Hit
CCCACCGATGCCTCCACCGAAGCTGGCGCTCCGGTTTCCAAGGCTCCTAC36690.13521775808293723No Hit
CACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCC36020.13274853219262467No Hit
GGCCTATTCACTGCGGCTCTCTCGGGCTTGCACCCTAACAGAGCACCCCT35300.13009503571348283No Hit
CCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCC35040.12913682865157047No Hit
GCCGGTATGAGTAGCGAAAGAGGGGTGAGAATCCCCTCCACCGAATGCCT34950.12880514159167775No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCCCTATGTGTAGATCT34940.1287682874739119Illumina Single End PCR Primer 1 (96% over 32bp)
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGAT34830.12836289217848743No Hit
CCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACT34400.1267781651145555No Hit
CTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACCGTCAAGGTACCG34280.12633591570136518No Hit
CGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGAT33830.12467748040190152No Hit
GCCGAGTTCCTTAACGAGAGTTCTCTCGATCACCTTAGGATTCTCTCCTC32770.12077094391872045No Hit
CTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTT32630.12025498626999842No Hit
CCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCAC32410.11944419567914953No Hit
GCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGC32030.11804373920404687No Hit
CCGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCA31630.1165695744934125No Hit
CCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCAC31290.1153165344893733No Hit
CCGACCCGCACGAAAGGCGCAACGATCTGGGCACTGTCTCAACGAGAGAC30380.11196280977268011No Hit
CGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCA30210.11133628977066051No Hit
CCGGGATGGACGCACCGCTGGTGTACCAGTTGTTCTGCCAAGGGCATCGC29980.11048864506204574No Hit
GTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCA29950.11037808270874817No Hit
CAGGGATCTTACTTCCTTGCGGAATGGGAAATCTCATCTTGAGGGGGGCT29780.10975156270672858No Hit
CCCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCAC29710.10949358388236755No Hit
GTTGCTTCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCA29470.10860908505598693No Hit
GGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGG29080.10717177446311842No Hit
GTCGCGGGTAACCTGCATCTTCACAGGTACTATAATTTCACCGAGTCTCT29040.107024357992055No Hit
TGCCAATCGAACCTGGAGATAGCTGGTTCTCTCCGAAATAGCTTTAGGGC28790.1061030050479085No Hit
CTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTA28710.10580817210578163No Hit
GTTTTTGCTAAACAGTCGCCTGGGCCTATTCACTGCGGCTCTCTCGGGCT27430.10109084503175166No Hit
CAGCCCTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGG27230.10035376267643448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCTC35050.067.8031445
TAGATCT36950.067.5372244
GTGTAGA41900.067.4940341
TGTAGAT41650.067.3109342
ATCTCGG35100.066.70940447
TCTATGT31450.066.6613736
ATGTGTA42900.066.65501439
GGTGGTC33300.066.63663552
TGGTCGC30300.066.4191454
CTCTATG31050.066.39291435
TCTCGGT33050.065.975848
ATCATTA25150.065.9642264
TATGTGT40550.065.9432838
CCGTATC23850.065.7442460
GTATCAT23950.065.6158762
CCTCTAT31150.065.39326534
GTGCCTC32150.065.31882531
GCCGTAT25250.065.28712559
GTCGCCG31650.064.9131156
GAGTGTG40250.064.8695727
Produced by FastQC (version 0.11.2)