FastQCFastQC Report
Thu 27 Jul 2017
000000000-B5WD2_l01n02_oat1_b.35200000098760.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B5WD2_l01n02_oat1_b.35200000098760.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1621087
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT61450.37906663861964224No Hit
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG45810.2825881646080685No Hit
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGA38440.23712484277524895No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT37200.22947565429862804Illumina Single End PCR Primer 1 (96% over 32bp)
CGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGAT31840.19641142023839558No Hit
CACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT30410.18759017868874403No Hit
CGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACA29960.18481426351577676No Hit
GCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCT29030.17907737215831107No Hit
CACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCC27050.16686334539725506No Hit
GCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAAGGCGCAACGAT26070.16081801902057075No Hit
CTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACCGTCAAGGTACCG25400.1566849897630417No Hit
TTCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCC25040.1544642576246679No Hit
GTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAG24950.15390907459007444No Hit
CCGGGATGGACGCACCGCTGGTGTACCAGTTGTTCTGCCAAGGGCATCGC24180.14915917529410821No Hit
GCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACAC23440.14459433700967314No Hit
CCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGA23030.14206516985208073No Hit
GGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGG22770.1404613077521441No Hit
CACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCC22430.1383639496214577No Hit
GCGAAATATAGTAGCGAAGTTCCTGATTCCACACTGCCAAGAAAAGCCTC22010.1357730954600216No Hit
GCCGGTATGAGTAGCGAAAGAGGGGTGAGAATCCCCTCCACCGAATGCCT21900.1350945384177407No Hit
CTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTT21860.13484779040236583No Hit
CGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCA21350.13170175320633626No Hit
GTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCA21230.13096150916021163No Hit
CCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCC20880.12880246402568152No Hit
CCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCATTC20790.12824728099108806No Hit
GGCCTATTCACTGCGGCTCTCTCGGGCTTGCACCCTAACAGAGCACCCCT19830.12232532862209124No Hit
GTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAA19800.12214026761056007No Hit
CTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGG19800.12214026761056007No Hit
GTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACC19790.12207858060671636No Hit
GGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGA19530.12047471850677972No Hit
CCCGGGTCTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCC19510.12035134449909228No Hit
CAGGGATCTTACTTCCTTGCGGAATGGGAAATCTCATCTTGAGGGGGGCT19360.11942603944143652No Hit
CCCACCGATGCCTCCACCGAAGCTGGCGCTCCGGTTTCCAAGGCTCCTAC19270.11887085640684306No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGAT19010.11726699430690642No Hit
CCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCA18720.11547807119543861No Hit
CGGGGCTAAACGTATCACCGAAGCTGCGGACTGTTCTTCGAACAGTGGTA17870.11023467586872265No Hit
GGCGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAA17810.10986455384566034No Hit
CCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACC17510.10801394373034882No Hit
GTTTTTGCTAAACAGTCGCCTGGGCCTATTCACTGCGGCTCTCTCGGGCT17380.10721201268038051No Hit
CGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGT17260.10647176863425592No Hit
CTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTA17080.105361402565069No Hit
CCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCAC17030.10505296754585042No Hit
CCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACT16510.10184524334597711No Hit
CCGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCA16380.10104331229600878No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG150.00222506970.0000117
TAGATCT6100.068.8524644
GTGTAGA6700.068.4328441
TGTAGAT6850.067.4452642
GGTGGTC5800.066.9827652
CCGTATC4250.065.88235560
ATCTCGG6400.065.62547
GTCGCCG5600.065.62556
AGATCTC6350.065.5905545
GTATCAT4650.064.73118662
GAGTGTG6750.064.29629527
TCTCGGT6000.064.16666448
AGTGTGC6600.064.16666428
TGGTCGC5600.063.7554
ATGTGTA7600.063.09210239
TCTATGT5700.062.6315836
CCTCTAT5550.062.43243434
CTCTATG5750.061.4782635
TATCATT4850.061.34020663
TCGCCGT5350.060.18691357
Produced by FastQC (version 0.11.2)