FastQCFastQC Report
Thu 27 Jul 2017
000000000-B5WD2_l01n02_79t2_n.352000000987b0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B5WD2_l01n02_79t2_n.352000000987b0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1582762
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT86410.5459443681362075Illumina Single End PCR Primer 1 (96% over 32bp)
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG63460.4009446777216031No Hit
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGA44200.27925866302071944No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT41350.2612521655182523No Hit
CACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT36110.22814548239090907No Hit
GCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCT31210.19718694282526367No Hit
CGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGAT30100.19017388590325013No Hit
GCGAAATATAGTAGCGAAGTTCCTGATTCCACACTGCCAAGAAAAGCCTC29950.18922617550838344No Hit
CACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCC29300.18511943046396112No Hit
GGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGG27810.17570550720828526No Hit
CGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACA25830.1631957299960449No Hit
CGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCA25110.15864672010068476No Hit
CCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGA24700.15605631168804912No Hit
GCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAAGGCGCAACGAT24440.1544136136702802No Hit
GTGGGTTCGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGG23690.14967506169594672No Hit
CAGCCCTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGG23690.14967506169594672No Hit
CCCGGGTCTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCC23240.14683193051134663No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGAT23100.14594740080947102No Hit
CCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCC23020.14544195526554213No Hit
CGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCA22800.14405198001973765No Hit
CCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCATTC22250.14057704190522644No Hit
GCCGGTATGAGTAGCGAAAGAGGGGTGAGAATCCCCTCCACCGAATGCCT22070.1394397894313864No Hit
GGCCTATTCACTGCGGCTCTCTCGGGCTTGCACCCTAACAGAGCACCCCT21610.1365334775537952No Hit
CCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCA21170.13375352706218624No Hit
CTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTT20990.13261627458834618No Hit
GCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACAC20760.1311631186495506No Hit
CACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCC20520.1296467820177639No Hit
GGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCA20380.12876225231588828No Hit
CGAATTCAGTCAAACTCCGAATGCCAATGACTTATCCTTGGGAGTCAGAC19950.12604548251727046No Hit
GCCGAGTTCCTTAACGAGAGTTCTCTCGATCACCTTAGGATTCTCTCCTC19840.1253504948943682No Hit
CCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCAC18890.11934832906021246No Hit
CCGGGATGGACGCACCGCTGGTGTACCAGTTGTTCTGCCAAGGGCATCGC18630.11770563104244351No Hit
CTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGG18200.11498886124382568No Hit
CCGACCCGCACGAAAGGCGCAACGATCTGGGCACTGTCTCAACGAGAGAC18090.11429387362092343No Hit
GTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAG17940.11334616322605673No Hit
CCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACT17770.1122720914452078No Hit
CCTGATTCCACACTGCCAAGAAAAGCCTCTAGCGAGGTGAGAGGTGCCCG17740.11208254936623448No Hit
CCGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTC17620.11132438105034112No Hit
GGCAAATCCGTTTCCCATAAGGCTGAGCTGTGATGGCGAGCGAAATATAG17440.11018712857650108No Hit
CCGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCA17400.10993440580453662No Hit
GGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGA16880.10664900976899874No Hit
TGGCGAGCGAAATATAGTAGCGAAGTTCCTGATTCCACACTGCCAAGAAA16750.10582766076011427No Hit
GGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCA16720.10563811868114094No Hit
GTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATA16650.10519585383020316No Hit
GGCGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAA16640.10513267313721204No Hit
GCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGC16540.10450086620730091No Hit
CAGGGATCTTACTTCCTTGCGGAATGGGAAATCTCATCTTGAGGGGGGCT16170.10216318056662972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGTC12000.067.37552
GTGTAGA14550.066.3917541
CTCTATG11050.066.199135
TCTATGT11250.065.9555636
TGTAGAT14450.065.64014442
TAGATCT13400.065.559744
ATGTGTA14600.065.4452139
ATCTCGG13000.064.8846147
AGATCTC13150.064.676845
GTGCCTC11400.064.4736931
GTCGCCG12200.064.262356
TGGTCGC12400.063.7903254
TCTCGGT13100.063.32061448
TATCATT10200.062.79411763
GTATCAT10450.062.63157762
GCCGTAT11050.062.39819359
GAGTGTG14550.061.34020227
AGTGTGC14550.061.34020228
CCTCTAT11950.061.2133934
CCGTATC11100.061.1711760
Produced by FastQC (version 0.11.2)