FastQCFastQC Report
Thu 27 Jul 2017
000000000-B5WD2_l01n02_79t2_b.35200000098754.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B5WD2_l01n02_79t2_b.35200000098754.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2583852
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG116850.45223178417339693No Hit
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGA70390.272422723902143No Hit
CCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCA59800.23143740430953477No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT57860.2239292343369512No Hit
CACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT56160.21734991013417176No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT53320.20635856852482265Illumina Single End PCR Primer 1 (96% over 32bp)
GCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCT52580.20349462740125984No Hit
CGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACA51550.199508330972517No Hit
GCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAAGGCGCAACGAT48980.18956194085419753No Hit
CCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACT46240.17895761831560011No Hit
CCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCC46210.17884151259437459No Hit
CCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCATTC46160.17864800305899875No Hit
CCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCAC45150.17473911044440626No Hit
GCGAAATATAGTAGCGAAGTTCCTGATTCCACACTGCCAAGAAAAGCCTC44010.17032709303783652No Hit
CGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCA43070.16668911377277026No Hit
GGCCTATTCACTGCGGCTCTCTCGGGCTTGCACCCTAACAGAGCACCCCT43040.16657300805154474No Hit
CGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGAT41400.16022589529121636No Hit
CACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCC39380.15240811006203142No Hit
GTGGGTTCGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGG38810.15020210135874654No Hit
CCCACCGATGCCTCCACCGAAGCTGGCGCTCCGGTTTCCAAGGCTCCTAC37530.1452482572531244No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGAT37140.14373888287719264No Hit
CTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTT36990.14315835427106507No Hit
GCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACAC36880.14273263329323815No Hit
GCCGAGTTCCTTAACGAGAGTTCTCTCGATCACCTTAGGATTCTCTCCTC36860.14265522947908782No Hit
CGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCA36010.1393655673776981No Hit
CGAATTCAGTCAAACTCCGAATGCCAATGACTTATCCTTGGGAGTCAGAC35160.1360759052763084No Hit
CACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCC34910.13510835759942907No Hit
CCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGA34810.13472133852867732No Hit
GGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCA34170.13224441647586627No Hit
CAGCCCTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGG34140.13212831075464074No Hit
GGCGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAA33430.12938047535230346No Hit
CCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCAC33130.12821941814004828No Hit
CCCGGGTCTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCC32930.12744537999854483No Hit
CCGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCA31830.12318817022027577No Hit
GGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGG31090.12032422909671298No Hit
TGGCGAGCGAAATATAGTAGCGAAGTTCCTGATTCCACACTGCCAAGAAA30930.1197049985835102No Hit
CCTGATTCCACACTGCCAAGAAAAGCCTCTAGCGAGGTGAGAGGTGCCCG30450.1178473070439019No Hit
GGGAAACAGCCCAGACCGCCAGCTAAGGTCCCAAAGTATACGTTAAGTGG29670.1148285582920384No Hit
GTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAG29630.11467375066373771No Hit
GCCGGTATGAGTAGCGAAAGAGGGGTGAGAATCCCCTCCACCGAATGCCT29030.11235163623922732No Hit
GGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTTCT28910.11188721335432525No Hit
GCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGC28450.11010692562886729No Hit
CTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGG28050.10855884934586035No Hit
TGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTTC28020.10844274362463485No Hit
GTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATA27570.10670115780625207No Hit
CCGACCCGCACGAAAGGCGCAACGATCTGGGCACTGTCTCAACGAGAGAC27460.10627543682842516No Hit
GTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGG26590.1029083709128851No Hit
GGCAAATCCGTTTCCCATAAGGCTGAGCTGTGATGGCGAGCGAAATATAG26550.1027535632845844No Hit
CTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTT26400.1021730346784568No Hit
CCGAGTTCCTTAACGAGAGTTCTCTCGATCACCTTAGGATTCTCTCCTCG26140.10116678509450232No Hit
TGCCAATCGAACCTGGAGATAGCTGGTTCTCTCCGAAATAGCTTTAGGGC26020.10070236220960024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA10550.064.02843541
TAGATCT9450.063.33333244
AGATCTC9200.062.01086845
GGTGGTC8700.061.1494352
TGTAGAT11050.061.1312242
ATGTGTA11300.061.017739
AGTGTGC9800.060.71428728
ATCTCGG9450.060.3703747
TCTATGT8650.059.88439636
GAGTGTG10250.059.75609627
CTCTATG8500.059.29411735
GTCGCCG8750.058.856
GTGCCTC8350.058.68263631
TCTCGGT9650.056.58030748
TGGTCGC8850.056.55367354
GTCGACG509.840733E-1055.99999656
CCGTATC7250.055.51724260
TGTAGGG12300.055.2032517
CCCTATG1600.054.68749635
CCTCTAT9100.054.23076634
Produced by FastQC (version 0.11.2)