000000000-B5WD2_l01n02_79t1_n.352000000987a4.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-B5WD2_l01n02_79t1_n.352000000987a4.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1919587
Sequences flagged as poor quality	0
Sequence length	76
%GC	50

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT	7252	0.3777895974498681	Illumina Single End PCR Primer 1 (96% over 32bp)
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG	5486	0.2857906414244314	No Hit
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGA	4843	0.2522938527922933	No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT	4170	0.21723422798758277	No Hit
CGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGAT	3806	0.19827181576036929	No Hit
GGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGG	3481	0.18134109055750014	No Hit
GCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCT	3095	0.16123259847040014	No Hit
CACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT	3079	0.16039908584502813	No Hit
CACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCC	3073	0.16008651861051362	No Hit
CGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACA	2763	0.1439372114939307	No Hit
CTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGG	2444	0.12731905352557607	No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGAT	2271	0.11830669826374111	No Hit
CTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTT	2183	0.11372237882419499	No Hit
CAGCCCTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGG	2181	0.1136181897460235	No Hit
CCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGA	2174	0.11325352797242323	No Hit
GCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACAC	2173	0.11320143343333748	No Hit
GGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGA	2101	0.1094506266191634	No Hit
CCCGGGTCTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCC	2039	0.10622076519584682	No Hit
GTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAA	1934	0.10075083859184294	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GGTGGTC	1145	66.0262	52
GTGTAGA	1350	65.07407	41
TAGATCT	1330	61.05263	44
ATCTCGG	1280	60.976562	47
AGATCTC	1265	60.592884	45
CTCTATG	1075	60.55814	35
GTCGCCG	1215	60.20576	56
GAGTGTG	1460	60.171238	27
TGTAGAT	1450	59.37931	42
GTGCCTC	1140	59.254387	31
AGTGTGC	1435	59.02439	28
ATGTGTA	1530	58.79085	39
TGGTCGC	1235	58.380566	54
TCTCGGT	1250	57.96	48
CCGTATC	1020	56.61765	60
TCTATGT	1155	56.363636	36
GTGCCCC	225	56.0	31
CCTCTAT	1170	55.64102	34
TGTAGGG	1690	54.674557	17
GTGTAGG	1765	52.747875	16

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content