FastQCFastQC Report
Thu 27 Jul 2017
000000000-B5WD2_l01n02_79t1_b.35200000098747.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B5WD2_l01n02_79t1_b.35200000098747.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2044001
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT353181.7278856517193484Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCCCTATGTGTAGATCT52690.25777873885580294Illumina Single End PCR Primer 1 (96% over 32bp)
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG50450.24681984010771035No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT43090.2108120299354061No Hit
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGA40480.1980429559476732No Hit
CGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACA35060.1715263348696992No Hit
GCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCT31200.15264180399128965No Hit
CACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCC29820.1458903395839826No Hit
GCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACAC29720.14540110303272846No Hit
CACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT29190.14280814931108154No Hit
CGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGAT28800.14090012676119043No Hit
GGCGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAA26870.13145786132198564No Hit
CCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGA26750.1308707774604807No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGAT24230.11854201636887654No Hit
GGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGG23820.11653614650873458No Hit
GCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAAGGCGCAACGAT22810.11159485734106783No Hit
GTGGGTTCGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGG20730.10141873707498186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGTC51550.067.5557752
AGATCTC54950.067.0700645
GTGTAGA61600.067.04545641
TAGATCT57850.066.12791444
ATGTGTA63500.066.031539
TGTAGAT61850.065.98221642
GTGCCTC48750.065.764131
AGTGTGC59550.065.53316528
TCTCGGT54750.065.52511648
CCCTATG8600.065.52325435
GTCGCCG51550.065.3831256
ATCTCGG56800.065.37852547
CTCTATG48650.065.251835
TCTATGT48650.065.17985536
TGGTCGC50700.065.1676554
GAGTGTG60700.065.1565127
CCGTATC43200.064.2476860
GTGCCCC9000.064.16666431
CCTCTAT49250.063.8883234
TCGCCGT48450.063.13725357
Produced by FastQC (version 0.11.2)