Basic Statistics
Measure | Value |
---|---|
Filename | 000000000-B5WD2_l01n01_vgt2_n.351000000987f7.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1510318 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 47 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT | 39403 | 2.6089207703278383 | TruSeq Adapter, Index 1 (97% over 36bp) |
CGCATTTTTAAATGGCGTACATGCCTCTTTTCATTCGGTAAAGAGGACTT | 2450 | 0.1622174932696293 | No Hit |
CCCAATAGTGCCGTTGCAGCTTTCCTTTGTTTTGAACCCGCTCTCAGCAG | 2169 | 0.14361213995992897 | No Hit |
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC | 2168 | 0.14354592873818628 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGACCTCGTAT | 2078 | 0.13758691878134274 | TruSeq Adapter, Index 1 (97% over 36bp) |
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT | 1927 | 0.12758902429819416 | No Hit |
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG | 1794 | 0.11878293180641428 | No Hit |
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG | 1730 | 0.11454541361488112 | No Hit |
GCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCC | 1598 | 0.10580553234484392 | No Hit |
CGGGGAGGTAGCGGTGCCCTGTACCTGCAATCCGCTCTAGCAGGGCCGAA | 1594 | 0.10554068745787311 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTATGCC | 5165 | 0.0 | 63.630203 | 47 |
TAGATCT | 4585 | 0.0 | 62.9771 | 39 |
GCGATAG | 5320 | 0.0 | 62.894737 | 35 |
CGTATGC | 5310 | 0.0 | 62.683617 | 46 |
AGATCTC | 4615 | 0.0 | 62.643555 | 40 |
GATAGAT | 4785 | 0.0 | 62.466038 | 37 |
ATAGATC | 4670 | 0.0 | 62.35546 | 38 |
CGATAGA | 5350 | 0.0 | 62.28037 | 36 |
TCGTATG | 5320 | 0.0 | 62.236843 | 45 |
CTCGTAT | 5215 | 0.0 | 62.08054 | 44 |
GTCACAG | 5500 | 0.0 | 61.599995 | 29 |
AGTCACA | 5505 | 0.0 | 61.54405 | 28 |
CACAGCG | 5490 | 0.0 | 61.52095 | 31 |
AGCGATA | 5475 | 0.0 | 61.305935 | 34 |
TATGCCG | 5375 | 0.0 | 61.013954 | 48 |
GCCGTCT | 5310 | 0.0 | 60.574387 | 51 |
GTCTTCT | 5385 | 0.0 | 59.860725 | 54 |
TCCAGTC | 5645 | 0.0 | 59.645706 | 25 |
ACTCCAG | 5705 | 0.0 | 59.57055 | 23 |
ATGCCGT | 5515 | 0.0 | 59.33817 | 49 |