FastQCFastQC Report
Thu 27 Jul 2017
000000000-B5WD2_l01n01_vgt2_n.351000000987f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B5WD2_l01n01_vgt2_n.351000000987f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1510318
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT394032.6089207703278383TruSeq Adapter, Index 1 (97% over 36bp)
CGCATTTTTAAATGGCGTACATGCCTCTTTTCATTCGGTAAAGAGGACTT24500.1622174932696293No Hit
CCCAATAGTGCCGTTGCAGCTTTCCTTTGTTTTGAACCCGCTCTCAGCAG21690.14361213995992897No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC21680.14354592873818628No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGACCTCGTAT20780.13758691878134274TruSeq Adapter, Index 1 (97% over 36bp)
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT19270.12758902429819416No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG17940.11878293180641428No Hit
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG17300.11454541361488112No Hit
GCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCC15980.10580553234484392No Hit
CGGGGAGGTAGCGGTGCCCTGTACCTGCAATCCGCTCTAGCAGGGCCGAA15940.10554068745787311No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC51650.063.63020347
TAGATCT45850.062.977139
GCGATAG53200.062.89473735
CGTATGC53100.062.68361746
AGATCTC46150.062.64355540
GATAGAT47850.062.46603837
ATAGATC46700.062.3554638
CGATAGA53500.062.2803736
TCGTATG53200.062.23684345
CTCGTAT52150.062.0805444
GTCACAG55000.061.59999529
AGTCACA55050.061.5440528
CACAGCG54900.061.5209531
AGCGATA54750.061.30593534
TATGCCG53750.061.01395448
GCCGTCT53100.060.57438751
GTCTTCT53850.059.86072554
TCCAGTC56450.059.64570625
ACTCCAG57050.059.5705523
ATGCCGT55150.059.3381749